PMID: 9165081Mar 1, 1997Paper

Purification and primary structure determination of a Bowman-Birk trypsin inhibitor from Torresea cearensis seeds

Biological Chemistry
Aparecida S TanakaC A Sampaio

Abstract

A Bowman-Birk-type trypsin inhibitor (TcTI) was purified from seeds of Torresea cearensis, a Brazilian native tree of the Papilionoideae sub-family of Leguminosae. Three forms of the inhibitor were separated by anion exchange chromatography. The major form with 63 amino acids was entirely sequenced; it shows a high structural similarity to the Bowman-Birk inhibitors from other Leguminosae. The putative reactive sites of the inhibitor are a lysine residue at position 15 and a histidine at position 42 as identified by alignment to related inhibitors, direct chemical modification and specific enzymatic degradation. Immunoprecipitation with antibodies raised in rats is reduced significantly if TcTI is complexed with chymotrypsin and, to a lesser degree, if complexed with trypsin. TcTI forms a ternary complex with trypsin and chymotrypsin. The binary complexes with trypsin or chymotrypsin were isolated by gel filtration. Dissociation constants of the complexes with trypsin, plasmin, chymotrypsin, and factor XIIa are 1, 36, 50, 1450 nM, respectively; human plasma kallikrein, human factor Xa, porcine pancreatic kallikrein and bovine thrombin are not inhibited. TcTI prolongs blood clotting time of the contact phase activation pathway b...Continue Reading

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Citations

Apr 8, 2003·Molecular Phylogenetics and Evolution·Marcia O MelloMarcio C Silva-Filho
Feb 21, 2008·BMC Plant Biology·Haining LinC Robin Buell
Mar 15, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Andrea ScirèRoberto Guerranti
Dec 12, 2007·Plant Biotechnology Journal·Naser FarrokhiJudy A Brusslan
Nov 26, 2005·Biological Chemistry·Adriana F de SouzaClaudio A M Sampaio
Jul 30, 2002·Journal of Biomolecular Structure & Dynamics·Arnd B E BrauerRobin J Leatherbarrow
Jul 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juliana Helena Castro E SilvaSilvia Lima Costa
May 10, 2005·Acta Biochimica Et Biophysica Sinica·Rui-Feng QiCheng-Wu Chi
Sep 5, 2020·European Journal of Medicinal Chemistry·Clara DavoineLionel Pochet
Aug 12, 1999·Biochemical and Biophysical Research Communications·N R BuenoM L Oliva

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