PMID: 6406228Jun 1, 1983Paper

Purification and properties of 2-aminoethylphosphonate:pyruvate aminotransferase from Pseudomonas aeruginosa

European Journal of Biochemistry
C DumoraA Cassaigne

Abstract

2-Aminoethylphosphonate aminotransferase has been purified to homogeneity with a yield of 15% from cell extracts of Pseudomonas aeruginosa. The molecular weight of the enzyme was estimated by gel filtration to be 65000 +/- 2000. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis yielded a molecular weight of 16500 +/- 1000, suggesting a tetrameric model for this protein. The absorption spectrum exhibits maxima at 280 nm, 335 nm and 415 nm which are characteristic of a pyridoxal-phosphate-dependent enzyme: 4 mol of pyridoxal 5'-phosphate/mol of enzyme have been found. This aminotransferase catalyzes the transfer of the amino group of 2-aminoethylphosphonate (ciliatine) to pyruvate to give 2-phosphonoacetaldehyde and alanine. A pH optimum between 8.5-9 and an activity increasing from 30 degrees C to 50 degrees C have been observed. The reaction follows Michaelis-Menten kinetics with Km values of 3.85 mM and 3.5 mM for ciliatine and pyruvate respectively. This enzyme shows a very high specificity since ciliatine and pyruvate are the only amino donor and acceptor respectively. Methyl, ethyl and propylphosphonic acids are better competitors towards ciliatine than their alpha-amino derivatives. 3-Aminopropylphosphonate, the hi...Continue Reading

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Citations

Aug 1, 1993·European Journal of Biochemistry·A M LacosteJ Vercauteren
Nov 24, 2007·Annual Review of Microbiology·Andrea K White, William W Metcalf
Oct 29, 2013·Current Microbiology·Magdalena Klimek-OchabEwa Zymańczyk-Duda
Jun 6, 2009·Annual Review of Biochemistry·William W Metcalf, Wilfred A van der Donk
Jul 11, 2002·Journal of Bacteriology·Alexander D KimBrian M Martin
Mar 21, 2021·Biochemical and Biophysical Research Communications·Haizhu JiaMark Bartlam
Apr 1, 1992·Journal of Bacteriology·K S LeeB L Wanner
Sep 1, 1992·Applied and Environmental Microbiology·E ZboińskaP Kafarski
Oct 28, 2016·Chemical Reviews·Geoff P Horsman, David L Zechel
Jan 21, 2014·Journal of Natural Products·Joel P CioniWilliam W Metcalf

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