Purification and properties of a chitinase from Penicillium sp. LYG 0704

Protein Expression and Purification
Yoon Gyo LeeHyeun-Jong Bae

Abstract

The chitinase producing Penicillium sp. LYG 0704 was procured from soil of the Chonnam National University crop field. The chitinase activity was detected after the first day which increased gradually and reached its maximum after 3 days of cultivation. The chitinase was purified from a culture medium by precipitation with isopropanol and column chromatography with Mono Q and Butyl-Sepharose. The molecular mass of chitinase was estimated to be 47 kDa by SDS-PAGE. Optimal pH and temperature were 5.0 and 40 degrees C, respectively. The N-terminal amino acid sequence of the enzyme was determined to be (1)AGSYRSVAYFVDWAI(15). The fully cloned gene, 1287 bp in size, encoded a single peptide of 429 amino acids. BLAST search of the chitinase gene sequence showed similarity with chitinase of Aspergillus fumigatus Af293 chitinase gene (58%) and A. fumigatus class V chitinase ChiB1 gene (56%).

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Citations

Aug 28, 2012·Current Microbiology·Maria Swiontek Brzezinska, Urszula Jankiewicz
Aug 31, 2013·Current Microbiology·Maria Swiontek BrzezinskaMaciej Walczak
Feb 19, 2013·Journal of Basic Microbiology·Xinqi HuangXingming Yang
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Feb 27, 2019·The Journal of Microbiology·Dawoon ChungJaejoon Jung
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Nov 16, 2016·AMB Express·Kailas D SonawaneShailesh R Waghmare
Aug 7, 2021·Biochimica Et Biophysica Acta. Proteins and Proteomics·Zhengqiang JiangShaoqing Yang

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Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

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Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.