Purification and properties of a NAD-linked 1,2-propanediol dehydrogenase from propane-grown Pseudomonas fluorescens NRRL B-1244

Archives of Biochemistry and Biophysics
C T HouI Barist

Abstract

NAD-dependent 1,2-propanediol dehydrogenase (EC 1.1.1.4) activity was detected in cell-free crude extracts of various propane-grown bacteria. The enzyme activity was much lower in 1-propanol-grown cells than in propane-grown cells of Pseudomonas fluorescens NRRL B-1244, indicating that the enzyme may be inducible by metabolites of propane subterminal oxidation. 1,2-Propanediol dehydrogenase was purified from propane-grown Ps. fluorescens NRRL B-1244. The purified enzyme fraction shows a single-protein band upon acrylamide gel electrophoresis and has a molecular weight of 760,000. It consists of 10 subunits of identical molecular weight (77,600). It oxidizes diols that possess either two adjacent hydroxy groups, or a hydroxy group with an adjacent carbonyl group. Primary and secondary alcohols are not oxidized. The pH and temperature optima for 1,2-propanediol dehydrogenase are 8.5 and 20-25 degrees C, respectively. The activation energy calculated is 5.76 kcal/mol. 1,2-Propanediol dehydrogenase does not catalyze the reduction of acetol or acetoin in the presence of NADH (reverse reaction). The Km values at 25 degrees C, pH 7.0, buffer solution for 1,2-propan1,2-propanediol dehydrogenase are 8.5 and 20-25 degrees C, respectively...Continue Reading

References

May 1, 1970·Journal of Bacteriology·R Y StanierA C Wilson
Jul 1, 1969·Journal of Bacteriology·J R Vestal, J J Perry
Jun 1, 1982·Archives of Biochemistry and Biophysics·C T HouN Barnabe
Nov 1, 1952·Journal of Bacteriology·S LEVINE, L O KRAMPITZ
May 1, 1954·The Biochemical Journal·E SCHRAME J BIGWOOD
Apr 15, 1961·Biochimica Et Biophysica Acta·E HUFF
Jan 1, 1962·Antonie van Leeuwenhoek·J W FOSTER
Jan 1, 1963·Biochimica Et Biophysica Acta·J N BAPTISTM J COON
May 1, 1963·Journal of Bacteriology·H B LUKINS, J W FOSTER
Jan 1, 1960·Archiv für Mikrobiologie·E R LEADBETTER, J W FOSTER
Sep 1, 1940·The Biochemical Journal·R A Kekwick

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