Purification and properties of a new beta-lactamase from Pseudomonas cepacia.

Antimicrobial Agents and Chemotherapy
K HiraiS Mitsuhashi

Abstract

An inducible beta-lactamase was purified from a beta-lactam antibiotic-resistant strain (GN11164) of Pseudomonas cepacia. The purified enzyme preparation gave a single protein band on polyacrylamide gel electrophoresis. The specific enzyme activity for the hydrolysis of cephalothin as a substrate was 314.5 U/mg of protein. The optimal pH was about 8.0, and the optimal temperature was 45 degrees C. The isoelectric point was 9.3, and the molecular weight was estimated to be about 22,000 to 24,000 from gel filtration on a Sephadex G-200 column and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme activity was inhibited by iodine, p-chloromercuribenzoate, clavulanic acid, CP 45899, and cloxacillin. The beta-lactamase showed a unique substrate profile by hydrolyzing most of the cephalosporins, including cefuroxime, cefotaxime (HR 756), ampicillin, and penicillin G, at a high rate.

References

Sep 1, 1977·Journal of Bacteriology·Y Fujii-KuriyamaS Sugawara
Nov 1, 1978·Antimicrobial Agents and Chemotherapy·H C Neu, K P Fu
Dec 1, 1979·Antimicrobial Agents and Chemotherapy·N MatsubaraS Mitsuhashi
Feb 1, 1978·Antimicrobial Agents and Chemotherapy·K TsuchiyaH Nagatomo
Oct 1, 1978·Antimicrobial Agents and Chemotherapy·K TsuchiyaT Nishi
Feb 9, 1976·Biochemical and Biophysical Research Communications·J W Dale, J T Smith
Jun 1, 1976·The Journal of Antimicrobial Chemotherapy·R B Sykes, M Matthew
Aug 1, 1977·Antimicrobial Agents and Chemotherapy·R LetarteD Allard-Leprohon
Jan 1, 1976·Journal of Clinical Microbiology·S M GelbartH B Greenlee
Apr 17, 1971·Lancet·D C SpellerA C Viant
Mar 1, 1973·Japanese Journal of Microbiology·S YaginumaS Mitsuhashi
Jun 1, 1974·The Biochemical Journal·S G Waley
Mar 1, 1974·Japanese Journal of Microbiology·S YaginumaS Mitsuhashi
Jul 1, 1973·Analytical Biochemistry·G W RossC H O'Callaghan
Nov 1, 1970·Journal of Bacteriology·T SawaiS Mitsuhashi
May 1, 1962·The Biochemical Journal·R P NOVICK

❮ Previous
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Citations

Jul 29, 2011·Biotechnology Letters·K Narayanan RajnishParamasamy Gunasekaran
May 1, 1986·The Journal of Pediatrics·D A Goldmann, J D Klinger
Apr 1, 1980·Antimicrobial Agents and Chemotherapy·K SatoS Mitsuhashi
Jul 1, 1980·Antimicrobial Agents and Chemotherapy·S MasuyoshiS Mitsuhashi
Jul 1, 1980·Antimicrobial Agents and Chemotherapy·S MinamiS Mitsuhashi
Nov 1, 1980·Antimicrobial Agents and Chemotherapy·M MatsuuraS Mitsuhashi
Dec 1, 1980·Antimicrobial Agents and Chemotherapy·S MinamiS Mitsuhashi
Jan 1, 1981·Antimicrobial Agents and Chemotherapy·N MatsubaraS Mitsuhashi
Aug 1, 1981·Antimicrobial Agents and Chemotherapy·K HiraiS Mitsuhashi
Apr 1, 1982·Antimicrobial Agents and Chemotherapy·F KobayashiS Mitsuhashi
Aug 1, 1982·Antimicrobial Agents and Chemotherapy·K KatsuS Mitsuhashi
Oct 1, 1982·Antimicrobial Agents and Chemotherapy·Y SainoS Mitsuhashi
Oct 1, 1982·Antimicrobial Agents and Chemotherapy·H MikamiS Mitsuhashi
Nov 1, 1982·Antimicrobial Agents and Chemotherapy·M TaiS Mitsuhashi
Mar 1, 1984·Antimicrobial Agents and Chemotherapy·Y SainoS Mitsuhashi
Nov 1, 1984·Antimicrobial Agents and Chemotherapy·J M LacroixF Malouin
Apr 1, 1985·Antimicrobial Agents and Chemotherapy·M TodaA Tachibana
Apr 1, 1985·Antimicrobial Agents and Chemotherapy·T FujiiS Mitsuhashi
Apr 1, 1985·Antimicrobial Agents and Chemotherapy·K SatoS Mitsuhashi
Aug 1, 1985·Antimicrobial Agents and Chemotherapy·C KliebeB Wiedemann
Apr 1, 1986·Antimicrobial Agents and Chemotherapy·E YokotaS Mitsuhashi
May 1, 1986·Antimicrobial Agents and Chemotherapy·T FujiiS Mitsuhashi
Nov 1, 1986·Antimicrobial Agents and Chemotherapy·F MalouinJ M Lacroix
Jul 1, 1987·Antimicrobial Agents and Chemotherapy·Y UtsuiS Mitsuhashi
Jun 1, 1988·Antimicrobial Agents and Chemotherapy·A PrinceN X Chin
Sep 1, 1988·Antimicrobial Agents and Chemotherapy·A TamuraS Mitsuhashi
Mar 1, 1989·Antimicrobial Agents and Chemotherapy·K Bush
Apr 1, 1990·Antimicrobial Agents and Chemotherapy·H MatsuiS Mitsuhashi
May 1, 1994·Antimicrobial Agents and Chemotherapy·N H Georgopapadakou, C McCaffrey
Jun 1, 1995·Antimicrobial Agents and Chemotherapy·K BushA A Medeiros
Sep 1, 1995·Antimicrobial Agents and Chemotherapy·N Matsumura, S Mitsuhashi
Oct 27, 2010·BMC Genomics·Karlene H LynchJonathan J Dennis
Mar 19, 2002·Antimicrobial Agents and Chemotherapy·Terence K M CheungP Y Chau
Mar 17, 2015·Expert Review of Anti-infective Therapy·Vikas GautamAmit Agarwal
Aug 12, 1998·Memórias do Instituto Oswaldo Cruz·M C de CastilloA P de Ruiz Holgado
Oct 1, 1995·Clinical Microbiology Reviews·D M Livermore

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