Purification and Properties of a Thermostable Pullulanase from a Newly Isolated Thermophilic Anaerobic Bacterium, Fervidobacterium pennavorans Ven5.

Applied and Environmental Microbiology
R KochGarabed Antranikian

Abstract

Extremely thermophilic anaerobic fermentative bacteria growing at temperatures between 50 and 80(deg)C (optimum, 65 to 70(deg)C) were isolated from mud samples collected at Abano Terme spa (Italy). The cells were gram-negative motile rods, about 1.8 (mu)m in length and 0.6 (mu)m in width, occurring singly and in pairs. Cells commonly formed spheroids at one end similar to Fervidobacterium islandicum and Fervidobacterium nodosum. The new isolate differs from F. nodosum by the 7% higher G+C content of its DNA (40.6 mol%) but is similar to Fervidobacterium pennavorans and F. islandicum in its G+C content and phenotypic properties. The phylogenetic dendrogram indicates that strain Ven5 belongs to the order Thermotogales and shows the highest 16S ribosomal DNA sequence similarity to F. pennavorans, F. islandicum, and F. nodosum, with similarities of 99.0, 98.6, and 96.0%, respectively. During growth on starch the strain produced a thermostable pullulanase of type I which preferentially hydrolyzed (alpha)-1,6 glucosidic linkages. The enzyme was purified 65-fold by anion-exchange, gel permeation, and hydrophobic chromatography. The native pullulanase has a molecular mass of 240,000 Da and is composed of three subunits, each with a mol...Continue Reading

References

Jun 1, 1979·Microbiological Reviews·W E BalchR S Wolfe
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Jan 1, 1970·European Journal of Biochemistry·J De LeyA Reynaerts
Apr 1, 1970·Archives of Biochemistry and Biophysics·M Abdullah, D French
Oct 15, 1993·FEMS Microbiology Letters·F A Rainey, E Stackebrandt
Nov 1, 1964·Analytical Biochemistry·T JOVINM A NAUGHTON
Apr 1, 1965·The Journal of Cell Biology·J M FRASCA, V R PARKS
Aug 1, 1996·Applied and Environmental Microbiology·A B Friedrich, G Antranikian
Jan 1, 1983·Systematic and Applied Microbiology·V A HussK H Schleifer
Jan 1, 1995·World Journal of Microbiology & Biotechnology·C Leuschner, G Antranikian

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Citations

Sep 22, 1999·European Journal of Biochemistry·R M SchwerdtfegerG Antranikian
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·C Vieille, G J Zeikus
Jun 9, 2004·Applied and Environmental Microbiology·Costanzo BertoldoWolfgang Liebl
Jan 10, 2008·Applied Microbiology and Biotechnology·Dorra Zouari AyadiSamir Bejar
Sep 20, 2012·Enzyme Research·Siew Ling HiiArbakariya Bin Ariff
Jun 25, 2010·Critical Reviews in Biotechnology·Chilukoti NeerajaAppa Rao Podile
Dec 3, 1999·Applied and Environmental Microbiology·J W LeeD L Kaplan
Mar 7, 2021·Science·Margaux M PinneyDaniel Herschlag
Sep 6, 2018·Extremophiles : Life Under Extreme Conditions·Scott WushkeRichard Sparling
Jun 1, 2002·Current Opinion in Chemical Biology·Costanzo Bertoldo, Garabed Antranikian
Jun 21, 2021·Biotechnology Advances·Wei XiaJing Wu
Mar 16, 2007·Microbial Cell Factories·Pernilla TurnerEva Nordberg Karlsson

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