Purification and properties of an acetylxylan esterase from Thermobifida fusca

Enzyme and Microbial Technology
Chao-Hsun Yang, Wen-Hsiung Liu

Abstract

An acetylxylan esterase from Thermobifida fusca NTU22 was purified 51-fold as measured by specific activity from crude culture filtrate by ultrafiltration concentration, Sepharose CL-6B and DEAE-Sepharose CL-6B column chromatography. The overall yield of the purified enzyme was 14.4%. The purified enzyme gave an apparent single protein band on an SDS-PAGE. The molecular mass of purified enzyme as estimated by SDS-PAGE and by gel filtration on Sepharose CL-6B was found to be 30 and 28kDa, respectively, indicating that the acetylxylan esterase from T. fusca NTU22 is a monomer. The pI value of the purified enzyme was estimated to be 6.55 by isoelectric focusing gel electrophoresis. The N-terminal amino acid sequence of the purified esterase was ANPYERGP. The optimum pH and temperature for the purified enzyme were 8.0 and 80°C, respectively. The Zn(2+), Hg(2+), PMSF and DIPF inhibited the enzyme activity. The K(m) value for p-nitrophenyl acetate and acetylxylan were 1.86μM and 0.15%, respectively. Co-operative enzymatic degradation of oat-spelt xylan by purified acetylxylan esterase and xylanase significantly increased the acetic acid liberation compared to the acetylxylan esterase action alone.

References

Jan 1, 1976·Advances in Carbohydrate Chemistry and Biochemistry·R F Dekker, G N Richards
Dec 1, 1990·Applied and Environmental Microbiology·K P McDermidC R MacKenzie
Jun 1, 1993·Enzyme and Microbial Technology·L P Christov, B A Prior
Mar 1, 1994·Applied and Environmental Microbiology·D IrwinD B Wilson
Apr 24, 1999·Applied and Environmental Microbiology·G DegrassiV Venturi
Oct 24, 2003·Biochimica Et Biophysica Acta·Peter BielySilvia Spániková
Dec 1, 1987·Applied and Environmental Microbiology·H LeeH Schneider
Dec 1, 1987·Applied and Environmental Microbiology·C R MackenzieK G Johnson
Jan 1, 1990·Applied and Environmental Microbiology·K P McDermidC W Forsberg
Aug 1, 1991·Applied and Environmental Microbiology·S L Bachmann, A J McCarthy
Mar 30, 2006·Journal of Biotechnology·Renato ChávezJaime Eyzaguirre
Jan 9, 2007·Journal of Bacteriology·Athanasios LykidisNikos Kyrpides
Jul 1, 1991·World Journal of Microbiology & Biotechnology·D C SmithT M Wood

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Citations

May 15, 2010·Applied Microbiology and Biotechnology·Susan BilligWolfgang Zimmermann
Aug 6, 2013·Folia Microbiologica·Elżbieta SzczyrbaGrażyna Bartelmus
Oct 7, 2015·Frontiers in Microbiology·L Shivlata, Tulasi Satyanarayana
May 15, 2012·Biotechnology Advances·Peter Biely
Oct 6, 2016·Enzyme and Microbial Technology·Fiyinfoluwa A AdesioyeDon A Cowan
Jan 1, 2010·International Journal of Molecular Sciences·Chao-Hsun YangYu-Chun Huang
Nov 16, 2019·World Journal of Microbiology & Biotechnology·Samkelo MalgasBrett I Pletschke
Jun 6, 2009·Journal of Agricultural and Food Chemistry·Chao-Hsun YangCheng-Yu Chen

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