PMID: 2105888Feb 14, 1990Paper

Purification and properties of an endo-1,4-xylanase excreted by a hydrolytic thermophilic anaerobe, Clostridium thermolacticum. A proposal for its action mechanism on larchwood 4-O-methylglucuronoxylan

European Journal of Biochemistry
Philippe DebeireM Vignon

Abstract

An extracellular xylanase from a thermophilic anaerobe, Clostridium thermolacticum, was purified 400-fold by ion-exchange chromatography and gel filtration. The purified enzyme had a specific activity of 31,670 nkat/mg of protein at 60 degrees C, a molecular mass of 39 kDa and a pI of 4.9. The enzyme exhibited maximal activity at 80 degrees C (1 h assay) and at pH 6.0-6.5. There was little loss of activity after 4 days at 60 degrees C and the enzyme was stable in the wide pH range 3-11. Examination of the hydrolysis products of larchwood xylan indicated that it was an endoxylanase; at the early stage of the reaction, xylose (Xyl)-containing oligosaccharides of 3-12 residues were released and after a prolonged incubation time, the neutral end-products were Xyl2 and Xyl3. Kinetic studies of the hydrolysis of xylose-containing oligosaccharides of 4-7 residues showed that the tetrasaccharide was hydrolysed more slowly than the pentasaccharide, while the calculated Km and V values for pentasaccharide and hexasaccharide were similar. The primary structures of the XylnGlcA produced by long-term hydrolysis of larchwood glucuronoxylan were determined on the basis of their carbohydrate composition, by methylation analysis and by 1H-NMR a...Continue Reading

References

Jan 1, 1976·Advances in Carbohydrate Chemistry and Biochemistry·R F Dekker, G N Richards
Sep 1, 1988·Microbiological Reviews·K K WongJ N Saddler
Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
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Apr 1, 1981·Analytical Biochemistry·J U Baenziger, M Natowicz

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Citations

Oct 23, 1997·Journal of Biotechnology·P BielyD Kluepfel
Jul 28, 1999·FEMS Microbiology Reviews·N KulkarniM Rao
Dec 29, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M TurkiewiczS Bielecki
Dec 5, 1991·European Journal of Biochemistry·J C Royer, J P Nakas
Apr 1, 1993·The International Journal of Biochemistry·L R Schofield, R M Daniel
Jan 1, 1997·Critical Reviews in Biotechnology·A Sunna, G Antranikian

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