PMID: 9546664Apr 18, 1998Paper

Purification and properties of an extremely thermostable membrane-bound sulfur-reducing complex from the hyperthermophilic Pyrodictium abyssi

European Journal of Biochemistry
R DirmeierKarl O Stetter

Abstract

The chemolithoautotrophic archaeon Pyrodictium abyssi isolate TAG 11 gains energy by reducing sulfur with H2 to H2S. From this hyperthermophile, a sulfur-reducing complex catalyzing this reaction was purified 13.5-fold. The native complex exhibited a brownish-yellow colour and showed an apparent molecular mass of 520 kDa. SDS/PAGE revealed the presence of nine different major polypeptides with apparent molecular masses of 82, 72, 65, 50, 47, 42, 40, 30 and 24 kDa. The native complex contained 50-55 mol acid-labile sulfur, 50-55 mol iron, 1.6 mol nickel, 1.2 mol copper, 2.8 mol cytochrome b and 0.3 mol cytochrome c (all per mol native complex). The temperature optimum of the H2:sulfur oxidoreductase complex was 100 degrees C, which is consistent with the physiological growth optimum of the native organism. The complex is extremely heat stable. During 5 h incubation at 100 degrees C, no decrease in H2S-forming activity could be observed.

Citations

Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ulf KüperHarald Huber
Mar 14, 2007·Archaea : an International Microbiological Journal·Kim BrüggerHans-Peter Klenk
Nov 8, 2015·Biochimica Et Biophysica Acta·Ana M P Melo, Miguel Teixeira
May 26, 2012·Environmental Microbiology·Yuchen LiuWilliam B Whitman
Oct 15, 2013·FEMS Microbiology Reviews·Florian Mayer, Volker Müller
Jun 27, 2018·FEMS Microbiology Reviews·Christopher T StraubRobert M Kelly
Oct 21, 2005·The Journal of Biological Chemistry·Marianne GuiralMarie-Thérèse Giudici-Orticoni
Mar 24, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Américo G DuarteInês A C Pereira
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·G SchäferV Müller

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