PMID: 6406224May 16, 1983Paper

Purification and properties of aryl acylamidase from Pseudomonas fluorescens ATCC 39004

European Journal of Biochemistry
P M HammondM D Scawen

Abstract

Aryl acylamidase has been purified from a strain of Pseudomonas fluorescens ATCC 39004, selected from soil on the basis of its ability to utilise acylanilide compounds as a sole source of carbon. The enzyme was purified to homogeneity by a combination of ion-exchange, hydrophobic and gel-permeation chromatography. A relative molecular mass of about 52 500 was estimated by gel filtration. The native enzyme was shown to be a monomeric protein by sodium dodecyl sulphate/polyacrylamide gel electrophoresis. The enzyme was maximally active at a pH of 8.6 and at a temperature of 45 degrees C. The enzyme shows Michaelis-Menten kinetics; Km values for nitroacetanilide (69 microM) and hydroxyacetanilide (6.1 microM) were low, indicating that the enzyme has a very high affinity for both substrates.

References

Jan 1, 1976·Comparative Biochemistry and Physiology. A, Comparative Physiology·B S Huja, S K Hong
May 6, 1975·European Journal of Biochemistry·J AltK Krisch
May 1, 1977·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·P G Douch, H M Gahagan
Jan 1, 1975·Journal of Agricultural and Food Chemistry·J M Tiedje, M J Hagedorn
Apr 1, 1975·Journal of General Microbiology·J Alt, K Krisch
Oct 27, 1975·Biochemical and Biophysical Research Communications·K P HsiungG G Guilbault
Nov 1, 1970·Journal of General Microbiology·M J Hynes, J A Pateman
Feb 14, 1981·Lancet·P M HammondC P Price
Nov 15, 1984·Analytical Biochemistry·P M HammondC P Price
May 1, 1960·The Biochemical Journal·R H NIMMO-SMITH
Feb 1, 1962·Journal of General Microbiology·M KELLY, P H CLARKE

❮ Previous
Next ❯

Citations

Jul 26, 2005·Applied Microbiology and Biotechnology·Yukie Akutsu-ShigenoToshiaki Nakajima-Kambe
Mar 28, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·S A ChubbC P Price
Feb 28, 1990·Clinica Chimica Acta; International Journal of Clinical Chemistry·H C MorrisC P Price
Jun 15, 1995·Clinica Chimica Acta; International Journal of Clinical Chemistry·C P PriceP M Hammond
Jul 1, 1991·European Journal of Biochemistry·H YoshiokaH Yamada
May 2, 2018·Nature Chemical Biology·Terence S CroftsGautam Dantas
Oct 23, 2008·Biotechnology and Bioengineering·Sonja HeumannGeorg M Guebitz
Feb 20, 2004·The Journal of Biological Chemistry·Michelle E PetersonRoy M Daniel
Nov 10, 2006·The Biochemical Journal·Michelle E PetersonRobert Eisenthal
Aug 1, 1991·Applied and Environmental Microbiology·D T VillarrealA Konopka
Nov 1, 1994·Applied and Environmental Microbiology·D T VillarrealA Konopka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.