Jan 1, 1975

Purification and properties of beta-galactosidase from Aspergillus oryzae

Journal of Biochemistry
Y TanakaT Horiuchi

Abstract

Beta-Galactosidase [EC 3.2.1.23] has been purified from a culture of Aspergillus oryzae by 2-propanol fractionation, column chromatography on DEAE-Sephadex A-50 and Sephadex G-200. The preparation was homogeneous on ultracentrifugation and disc electrophoresis. The enzyme showed pH optima of 4.5 with ONPG-1 as a substrate and 4.8 with lactose as a substrate. The stable pH range was from 4.0 to 9.0 and the optimum temperature was 46 degrees. The Michaelis constants were 7.2 X 10-minus 4 M with ONPG and 1.8 X 10-minus 2 M with lactose. Hg-2+, Cu-2+, N-bromosuccinimide, and sodium laurylsulfate caused marked inhibition. The apparent molecular weight was calculated to be about 105,000 by Sephadex gel filtration and sucrose density gradient centrifugation.

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Mentioned in this Paper

Mercury
Centrifugation, Density Gradient
Sephadex G 200
GLB1
Aspergillus flavus var. oryzae
DEAE-Sephadex A-50
Copper
Gel Chromatography
Electrophoresis, Disc
Hydrolase

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