Purification and properties of bovine nasal hyaline cartilage collagenase

The International Journal of Biochemistry
A Boukla


1. Collagenase from bovine nasal hyaline cartilage was extracted with 1 and 3 M NaCl in Tris-CaCl2 buffer. 2. Two peaks of collagenase activity were revealed on DE52 ion exchange column, collagenase 1 and collagenase 2. 3. The apparent mol. wt of collagenase 1 and 2 as determined by SDS-PAGE were 68 and 43 kDa, respectively. 4. Both enzymes degrade native collagen type II into two characteristic products, TCA(3/4) and TCB(1/4), at 25 degrees C and pH 7.6. 5. Trypsin and aminophenylmercuric acetate were capable of increasing the collagenase 1 activity. 6. The two enzymes can be characterized as metalloproteinases since they were inhibited by EGTA and 1,10-phenanthroline. The use of proteinase inhibitors (N-ethylmaleimide, iodoacetic acid, phenylmethylsulphonyl fluoride, soybean trypsin inhibitor, pepstatin, dithiothreitol) showed that the enzymes do not contain serine, cysteine or aspartic acid in their active sites.


Mar 1, 1978·Biochimica Et Biophysica Acta·C A VaterE D Harris
May 1, 1977·The Biochemical Journal·A SellersJ J Reynolds
Mar 1, 1978·The Biochemical Journal·P J RoughleyA J Barrett
Jul 1, 1988·Archives of Biochemistry and Biophysics·C W LinC H Evans
Jul 22, 1985·FEBS Letters·J DumasB Keil
May 1, 1969·The Journal of Investigative Dermatology·A Z Eisen
Aug 1, 1973·Analytical Biochemistry·M B BermanP F Davison
Sep 12, 1974·The New England Journal of Medicine·E D Harris, S M Krane
Sep 19, 1974·The New England Journal of Medicine·E D Harris, S M Krane
Sep 26, 1974·The New England Journal of Medicine·E D Harris, S M Krane
Jun 15, 1974·Experientia·K E KuettnerN Sorgente
Nov 15, 1984·European Journal of Biochemistry·K OtsukaH F Limeback
Dec 1, 1984·Calcified Tissue International·K OtsukaH F Limeback
Jan 1, 1983·Arthritis and Rheumatism·J P PelletierJ F Woessner
Mar 1, 1984·Collagen and Related Research·S M WilhelmR L Miller
Jul 1, 1980·Experimental Cell Research·N Sorgente, C K Dorey

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