Purification and properties of buffalo (Bubalus bubalis) erythrocyte hexokinase

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
M de C CesarR Rosa

Abstract

Buffalo erythrocytes contain one isozyme of hexokinase that apparently lacks microheterogeneity as shown by chromatographic properties. A single protein band was detected by means of Western blotting using an antibody raised in rabbits against homogeneous rat brain hexokinase I. The native protein has a molecular weight of 200,000 +/- 2880 by gel filtration. Partial purification of erythrocyte hexokinase by a combination of several procedures, including affinity chromatography, which was previously applied successfully to the purification of other mammalian type I hexokinases, produced a partially purified enzyme that showed several contaminants after SDS-polyacrylamide gel electrophoresis. The affinity of buffalo erythrocyte hexokinase for glucose (K(m) = 0.012 +/- 0.001 mM) is lower than most other mammal hexokinases type I. It phosphorylates other sugars, with considerably higher K(m) values. This isozyme is able to use MgATP but does not use MgGTP, MgCTP or MgUTP. We used inhibition patterns, obtained with products to elucidate enzyme sequential mechanisms. Our results are clearly in agreement with a random sequential mechanism and in disagreement with an ordered sequential mechanism with either glucose or ATP as the obliga...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·W J O'Sullivan, G W Smithers
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1976·Archives of Biochemistry and Biophysics·J P Casazza, H J Fromm
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Kalomenopoulou, I Beis
Feb 1, 1986·Molecular and Cellular Biochemistry·G SerafiniG Forniani
Oct 5, 1973·European Journal of Biochemistry·J S Easterby, M J O'Brien
Oct 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·H M Katzen, R T Schimke
Nov 1, 1967·Proceedings of the Society for Experimental Biology and Medicine·D J Galton, A E Jones
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
Oct 1, 1983·Archives of Biochemistry and Biophysics·M MagnaniG Fornaini
Jun 19, 1984·Biochimica Et Biophysica Acta·M MagnaniG Fornaini
Oct 15, 1982·Archives of Biochemistry and Biophysics·M Baijal, J E Wilson
Dec 10, 1982·Molecular and Cellular Biochemistry·G FornainiV Stocchi
Jan 1, 1995·Reviews of Physiology, Biochemistry and Pharmacology·J E Wilson
May 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J E Wilson, D A Schwab
Jan 7, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M de C CesarR Rosa

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