PMID: 6972868Feb 1, 1981Paper

Purification and properties of bullfrog prolactin

Endocrinologia Japonica
K Yamamoto, Sakaé Kikuyama

Abstract

Prolactin was purified from bullfrog adenohypophyses by extraction of acetone-dried powder with acid acetone and chromatography on DEAE-cellulose and Sephadex G100. The bullfrog prolactin had a molecular weight of 23,000 as determined by SDS gel electrophoresis. The isoelectric point was 5.76 when determined by electrofocussing. The amino acid composition of the purified prolactin closely resembles that of mammalian prolactin. The prolactin was found to be 3.6 times as potent as bovine prolactin in promoting collagen synthesis in the tadpole tail fin.

Citations

Dec 31, 2003·General and Comparative Endocrinology·Toshiharu YamamotoSakaé Kikuyama
Feb 17, 2001·General and Comparative Endocrinology·N TakahashiS Kikuyama
Feb 12, 2011·General and Comparative Endocrinology·Sakae Kikuyama, Kazuyoshi Tsutsui
Feb 1, 1988·General and Comparative Endocrinology·S Garcia-NavarroF Gracia-Navarro
Jul 1, 1990·General and Comparative Endocrinology·K MatsudaS Kikuyama
Jan 1, 1990·General and Comparative Endocrinology·K MatsudaS Kikuyama
Nov 1, 1987·General and Comparative Endocrinology·K Kubokawa, S Ishii
Dec 1, 1985·General and Comparative Endocrinology·Y S Chang, H Papkoff
Apr 1, 1991·General and Comparative Endocrinology·S C BrownS Kikuyama
Apr 1, 1991·General and Comparative Endocrinology·T Kobayashi, S Kikuyama
Aug 1, 1991·General and Comparative Endocrinology·A YasudaH Kawauchi
Jun 1, 1988·General and Comparative Endocrinology·K MiyajimaT Hirano
May 1, 1986·General and Comparative Endocrinology·K YamamotoS Kikuyama
Mar 1, 1989·General and Comparative Endocrinology·T KobayashiY Yokoo
Aug 1, 1985·General and Comparative Endocrinology·T HiranoS Ishii
Nov 1, 1990·General and Comparative Endocrinology·J M JossR M Dores
Sep 15, 2004·General and Comparative Endocrinology·Itaru HasunumaSakae Kikuyama
Mar 17, 2007·Critical Reviews in Toxicology·Douglas J FortLeslie W Touart
Jan 10, 2001·Canadian Journal of Physiology and Pharmacology·S KikuyamaA M Polzonetti-Magni
Jan 2, 2021·Molecular and Cellular Endocrinology·Sakae KikuyamaReiko Okada
Jul 1, 1986·General and Comparative Endocrinology·K YamamotoS Kikuyama

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