Purification and properties of carnitine acetyltransferase from rat liver

Journal of Biochemistry
S MiyazawaT Hashimoto

Abstract

Carnitine acetyltransferase was purified from rat liver after induction of the enzyme by feeding with di(2-ethylhexyl)phthalate. Two enzyme sources were used: the mitochondrial fraction and the homogenate of the liver. The purification procedure was essentially the same for the two enzyme sources. The enzyme purified from the mitochondrial fraction consisted of two different polypeptides with molecular weights of 36,500 and 27,000, whereas that from the homogenate consisted of one polypeptide with a molecular weight of 67,500. Amino acid compositions and peptide maps of the limited proteolytic products of the two enzyme preparations were nearly the same. Their antibodies were cross-reactive. Catalytic properties of the two preparations were nearly the same: the specific enzyme activities, double reciprocal plots of initial velocity study, substrate specificities for acylcarnitines having various carbon chain lengths, apparent Michaelis constants for substrates. On electrophoresis of the immunoprecipitate obtained after incubation of the mitochondrial extract, the two immunoreactive polypeptides with molecular weights of 36,500 and 27,000 were found. But only one polypeptide, with molecular weight of 67,500, was detected when th...Continue Reading

Citations

Aug 3, 2004·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·Sushma A Mengi, Naranjan S Dhalla
May 25, 2004·The Journal of Biological Chemistry·Antonio G CordenteDolors Serra
Jan 1, 1985·Teratogenesis, Carcinogenesis, and Mutagenesis·S JarmerR D Harbison
Jan 1, 1990·Journal of Inherited Metabolic Disease·R J WandersJ M Tager
May 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D R FellerG Krishna
May 24, 2011·Biochemical Society Transactions·Khosrow KashfiGeorge A Cook
May 20, 1990·European Journal of Biochemistry·W BloisiS Didonato
Aug 1, 1996·Applied Biochemistry and Biotechnology·K Jernejc, M Legisa
Feb 4, 2003·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Mario MateraVincenzo Savica
Oct 16, 1991·Biochimica Et Biophysica Acta·E P BrassO W Griffith
Nov 1, 1989·Archives of Biochemistry and Biophysics·H Hayashi, A Miwa
Feb 13, 1986·Biochemical and Biophysical Research Communications·S MiuraM Mori

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