PMID: 3214177Dec 1, 1988Paper

Purification and properties of catechol 1,2-dioxygenase (pyrocatechase) from Pseudomonas putida mt-2 in comparison with that from Pseudomonas arvilla C-1

Archives of Biochemistry and Biophysics
C NakaiM Nozaki

Abstract

Catechol 1,2-dioxygenase (pyrocatechase) has been purified to homogeneity from Pseudomonas putida mt-2. Most properties of this enzyme, such as the absorption spectrum, iron content, pH stability, pH optimum, substrate specificity, Km values, and amino acid composition, were similar to those of catechol 1,2-dioxygenase obtained from Pseudomonas arvilla C-1 [Y. Kojima et al. (1967) J. Biol. Chem. 242, 3270-3278]. These two catechol 1,2-dioxygenases were also found, from the results of Ouchterlony double diffusion, to share several antigenic determinants. The molecular weight of the putida enzyme was estimated to be 66,000 and 64,000 by sedimentation equilibrium analysis and Sephadex G-200 gel filtration, respectively. The enzyme gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, corresponding to Mr 32,000. The NH2-terminal sequence, which started with threonine, was determined up to 30 residues by Edman degradation. During the degradation, a single amino acid was released at each step. The NH2-terminal sequence up to 20 residues was identical to that of the beta subunit of the arvilla enzyme, with one exception at step 16, at which arginine was observed instead of glutamine. The COOH-terminal residu...Continue Reading

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Citations

Nov 6, 2003·International Journal of Biological Macromolecules·Y SanakisH Stamatis
Dec 1, 2005·Journal of the American Chemical Society·Geoff P HorsmanLindsay D Eltis
May 26, 2009·Journal of Bacteriology·Beatriz CámaraDietmar H Pieper
Jan 1, 1996·Annual Review of Microbiology·C S Harwood, R E Parales
Mar 28, 2012·Bioscience, Biotechnology, and Biochemistry·Mayu EnyaKiyofumi Maruyama
Jul 25, 2012·Journal of Biotechnology·Tino PolenMichael Bott
Jun 8, 2012·Water Research·Do Gyun LeeKung-Hui Chu
Jul 6, 2007·Biochemical and Biophysical Research Communications·Young-Ho YoonSeung Il Kim
Jun 8, 2017·FEMS Microbiology Letters·Alpa ChoudharyPrashant S Phale
Jan 1, 1989·Critical Reviews in Biotechnology·F S Sariaslani
Jun 1, 1990·Applied and Environmental Microbiology·Y P Chen, C R Lovell
Jul 1, 1995·Applied and Environmental Microbiology·M LatusF Lingens
Aug 1, 1997·Journal of Bacteriology·S I KimK S Ha

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