Mar 16, 1976

Purification and properties of cyclopentanone oxygenase of Pseudomonas NCIB 9872

European Journal of Biochemistry
M Griffin, P W Trudgill


1. Cyclopentanone oxygenase from Pseudomonas NCIB 9872 has been purified some 40-fold. It gives a single peak in the ultracentrifuge and a single major protein band on polyacrylamide gels contaminated with about 5% of a slower migrating impurity. Flavin dissociates from the protein during electrophoresis. 2. The enzyme has a molecular weight of about 200000 and is a homopolymeric assemblage of either three of four subunits of molecular weight 54000-58000. 3. The prosthetic group is FAD and values of about 2.5 are typically obtained for the number of moles bound to each mole of holoenzyme. Some FAD probably dissociates during purification and it seems likely that each subunit binds one FAD in the undamaged protein.

Mentioned in this Paper

Pseudomonas Infections
Polyacrylamide Gels
Hydatidiform Mole
Spectrophotometry, Ultraviolet
Binding (Molecular Function)
Purification Aspects

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