Purification and properties of cytochrome P-450 from homogenates of human fetal livers

Archives of Biochemistry and Biophysics
M KitadaY Kanakubo


A form of cytochrome P-450, namely P-450HFLa of human fetal livers, was purified to a specific content of 12.6 nmol/mg protein. The cytochrome P-450 preparation was electrophoretically homogeneous and had an apparent monomeric molecular weight of 51,000 as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The cytochrome showed catalytic activities as oxidations of N-methylaniline, ethylmorphine, N,N-dimethylaniline, N,N-dimethylnitrosamine, benzphetamine, aminopyrine, aniline, p-nitroanisole, and 7-ethoxycoumarin to various extents. In fetal liver homogenate, the amount of cytochrome P-450 that reacted with the antiserum to P-450HFLa accounted for more than 36% of the total cytochrome P-450 in three different fetal livers. On the other hand, the amount of P-450HFLa was less than 5% of the total cytochrome P-450 in adult liver microsomes.


Jun 6, 1977·Biochemical and Biophysical Research Communications·E F Johnson, U Muller-Eberhard
Apr 1, 1978·Analytical Biochemistry·A Aitio
Feb 9, 1976·Biochemical and Biophysical Research Communications·C Hashimoto, Y Imai
Oct 22, 1970·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·S J YaffeS Orrenius
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Jan 1, 1980·Archives of Biochemistry and Biophysics·P WangF P Guengerich
Jul 1, 1980·Clinical Pharmacokinetics·M R JuchauC J Omiecinski


Sep 1, 1992·Biomedical Chromatography : BMC·M IsaG Atassi
Mar 10, 2000·Biochemical and Biophysical Research Communications·E InoueT Kamataki
Jul 22, 1992·Biochemical Pharmacology·C M HuntG M Stave
Jan 1, 1988·Pharmacology & Therapeutics·P H Beaune, F P Guengerich
Jan 1, 1990·Pharmacology & Therapeutics·D E Ryan, W Levin
Sep 1, 1989·Mutation Research·M KitadaT Kamataki
Jun 1, 1993·Journal of Pharmacological and Toxicological Methods·J L Raucy, S J Carpenter
Feb 9, 2000·Pharmacology & Therapeutics·J A RingD J Morgan
Nov 4, 2009·Tissue Engineering. Part C, Methods·Alexander RingToshio Miki
May 1, 1994·British Journal of Clinical Pharmacology·C CazeneuveG Olive
Dec 5, 1991·European Journal of Biochemistry·J M TreluyerT Cresteil
Dec 1, 1990·Risk Analysis : an Official Publication of the Society for Risk Analysis·N J Gorelick
Jul 1, 1998·Pharmacology & Toxicology·J HakkolaO Pelkonen
May 1, 2002·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Hiroshi Yamazaki, T Kamataki
Sep 1, 2008·Interdisciplinary Toxicology·Lucie Bořek-DohalskáMarie Stiborova
Feb 18, 2015·Drug Metabolism Reviews·Jolantha BeyerleCornelia M Ulrich
Jun 27, 2009·Pharmacology & Therapeutics·Ilia G DenisovStephen G Sligar
May 1, 1991·Toxicology and Applied Pharmacology·H S RamsdellD L Eaton
May 4, 2004·Biochemical and Biophysical Research Communications·Tamihide MatsunagaShigeru Ohmori
Jan 4, 2001·Drug Metabolism Reviews·S A WrightonP B Watkins
Jan 1, 1994·Drug Metabolism Reviews·M Kitada, T Kamataki
Jan 1, 1989·Drug Metabolism Reviews·J E Hulla, M R Juchau
Oct 23, 2004·Drug Metabolism and Pharmacokinetics·Satoshi YamaoriT Kamataki
Jul 3, 2007·Orvosi hetilap·Krisztián BácsiPéter Lakatos
Dec 1, 1991·Japanese Journal of Cancer Research : Gann·K Kawajiri, Y Fujii-Kuriyama
Apr 1, 1991·Japanese Journal of Cancer Research : Gann·M KitadaT Kamataki
Jul 13, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Martin A HayesTommy B Andersson
Jan 1, 1992·Critical Reviews in Toxicology·S A Wrighton, J C Stevens
Apr 3, 2018·Biological & Pharmaceutical Bulletin·Yukihiro NomuraTsuyoshi Murai
Apr 29, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M S OggG G Gibson
Dec 2, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·T Kamataki
Jun 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D RatanasavanhA Guillouzo
Sep 1, 1987·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·T Cresteil
Jul 31, 1990·European Journal of Biochemistry·F MorelA Guillouzo
Apr 18, 2002·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·G G GibsonW El-Sankary
Dec 21, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Nao TorimotoMitsukazu Kitada
May 4, 2010·Journal of Pediatric Endocrinology & Metabolism : JPEM·Hui WangJiang Yue
Mar 19, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Hiroyoshi NakamuraMitsukazu Kitada
Feb 1, 2020·Archives of Biochemistry and Biophysics·Toshiro NiwaShoko Sasaki

Related Concepts

Cytochrome P-450 Oxygenase
Fetal Structures
Mixed Function Oxygenases
Microsomes, Liver

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.