Sep 27, 1978

Purification and properties of DNA polymerase-beta from guinea pig liver

Biochimica Et Biophysica Acta
T A KunkelR R Meyer

Abstract

Deoxyribonucleic acid polymerase-beta (EC 2.7.7.7) has been purified over 100 000-fold from a whole cell extract of guinea pig liver. The enzyme yields a single stainable band when subjected to non-denaturing polyacrylamide gel electrophoresis, and this band corresponds to the DNA polymerase activity when a sister gel is sliced and assayed. The final fraction has a specific activity of 21 000 units/mg; this value can be increased significantly by addition of various components, including glycols, polyamines or any of several protein factors which can be purified from the crude extract. The DNA polymerase-beta lacks detectable exonuclease or endonuclease activity, has an alkaline pH optimum and has a requirement for all four deoxyribonucleoside triphosphates, a divalent cation and a primer-template for maximal activity. While activated DNA is the preferred primer-template, the enzyme is capable of utilizing native and denatured DNA as well as several synthetic polynucleotides as primer-templates. The latter are especially effective when manganese is the divalent cation. Magnesium, at 10 mM, is the preferred divalent cation when activated DNA is used. Manganese, and to a lesser extent cobalt, can substitute for magnesium while zi...Continue Reading

  • References14
  • Citations9

Mentioned in this Paper

Cations, Divalent
Calcium [EPC]
Polymerase
Heparin
Magnesium Measurement
Calcium
Cavia
Endonuclease Activity
SDS-PAGE
Deoxyribonucleosides

About this Paper

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