Get paper from

journal cover

Purification and properties of Escherichia coli dihydrofolate reductase

Biochemistry

Dec 2, 1975

D P BaccanariJ J Burchall

Get paper from

Abstract

Dihydrofolate reductase has been purified 40-fold to apparent homogeneity from a trimethoprim-resistant strain of Escherichia coli (RT 500) using a procedure that includes methotrexate affinity column chromatography. Determinations of the molecular weight of the enzyme based on its amin...read more

Mentioned in this Paper

Alkalescens-Dispar Group
Chromatography, Affinity
Escherichia Coli Infections
Hydrogen-Ion Concentration
Protein Conformation
Tetrahydrofolate Dehydrogenase
Osmolality
Slow-K
Sodium Chloride, (24)NaCl
Methotrexate, (DL)-Isomer
79
Paper Details
References
    • References
    • Citations87

      References currently unavailable

      We're still populating references for this paper, please check back later.
    • References
    • Citations87
  • Purification and properties of Escherichia coli dihydrofolate reductase

    Biochemistry

    Dec 2, 1975

    D P BaccanariJ J Burchall

    PMID: 46

    DOI: 10.1021/bi00695a006

    Abstract

    Dihydrofolate reductase has been purified 40-fold to apparent homogeneity from a trimethoprim-resistant strain of Escherichia coli (RT 500) using a procedure that includes methotrexate affinity column chromatography. Determinations of the molecular weight of the enzyme based on its amin...read more

    Mentioned in this Paper

    Alkalescens-Dispar Group
    Chromatography, Affinity
    Escherichia Coli Infections
    Hydrogen-Ion Concentration
    Protein Conformation
    79

    Related Papers

    Paper Details
    References
    • References
    • Citations87

      References currently unavailable

      We're still populating references for this paper, please check back later.
    • References
    • Citations87
  • Get paper from

    © 2019 Meta ULC. All rights reserved.
    /papers/purification-and-properties-of-escherichia-coli/46