Purification and Properties of Extracellular Amylase from the Hyperthermophilic Archaeon Thermococcus profundus DT5432.

Applied and Environmental Microbiology
Y C ChungT Kudo

Abstract

A hyperthermophilic archaeon, Thermococcus profundus DT5432, produced extracellular thermostable amylases. One of the amylases (amylase S) was purified to homogeneity by ammonium sulfate precipitation, DEAE-Toyopearl chromatography, and gel filtration on Superdex 200HR. The molecular weight of the enzyme was estimated to be 42,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The amylase exhibited maximal activity at pH 5.5 to 6.0 and was stable in the range of pH 5.9 to 9.8. The optimum temperature for the activity was 80(deg)C. Half-life of the enzyme was 3 h at 80(deg)C and 15 min at 90(deg)C. Thermostability of the enzyme was enhanced in the presence of 5 mM Ca(sup2+) or 0.5% soluble starch at temperatures above 80(deg)C. The enzyme activity was inhibited in the presence of 5 mM iodoacetic acid or 1 mM N-bromosuccinimide, suggesting that cysteine and tryptophan residues play an important role in the catalytic action. The amylase hydrolyzed soluble starch, amylose, amylopectin, and glycogen to produce maltose and maltotriose of (alpha)-configuration as the main products. Smaller amounts of larger maltooligosaccharides were also produced with a trace amount of glucose. Pullulan; (alpha)-, (beta)-, and (gamma)-...Continue Reading

References

Feb 1, 1964·Archives of Biochemistry and Biophysics·J ROBYT, D FRENCH

❮ Previous
Next ❯

Citations

Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·C Vieille, G J Zeikus
Mar 3, 2007·International Journal of Systematic and Evolutionary Microbiology·Yi-Ju ChouWen-Ming Chen
Aug 5, 2008·International Journal of Systematic and Evolutionary Microbiology·Jui-Hsing ChouWen-Ming Chen
Jan 8, 2009·International Journal of Systematic and Evolutionary Microbiology·Jui-Hsing ChouWen-Ming Chen
Jul 25, 2009·International Journal of Systematic and Evolutionary Microbiology·A B ArunChiu-Chung Young
Jul 23, 2009·International Journal of Systematic and Evolutionary Microbiology·Shih-Yi SheuWen-Ming Chen
Oct 13, 2009·International Journal of Systematic and Evolutionary Microbiology·Peter KämpferA B Arun
Oct 13, 2009·International Journal of Systematic and Evolutionary Microbiology·Jiunn-Nan ChuChiu-Chung Young
May 10, 2011·International Journal of Systematic and Evolutionary Microbiology·C X YangY L Zhao
Jan 24, 2012·International Journal of Systematic and Evolutionary Microbiology·Zhan-Bin SunYong-Jun Feng
May 29, 2013·Journal of Basic Microbiology·Balu Jancy Kalpana, Shunmugiah Karutha Pandian
Jun 21, 2014·International Journal of Microbiology·Koel SahaSuvroma Gupta
Jan 28, 2016·International Journal of Environmental Health Research·Marisela Y Soto-PadillaMaría Isabel Estrada-Alvarado
Dec 3, 2016·International Journal of Systematic and Evolutionary Microbiology·Myong Chol KimFang Peng
Dec 9, 2008·International Journal of Systematic and Evolutionary Microbiology·Yi-Ju ChouWen-Ming Chen
Oct 11, 2005·Biotechnology Letters·Iderval da Silva SobrinhoCirano José Ulhoa
Jul 23, 2009·International Journal of Systematic and Evolutionary Microbiology·A B ArunShiawhwa Paul Su
Apr 10, 2008·International Journal of Systematic and Evolutionary Microbiology·Chiu-Chung YoungPeter Kämpfer
Nov 6, 2007·International Journal of Systematic and Evolutionary Microbiology·Jui-Hsing ChouWen-Ming Chen
Jul 23, 2009·International Journal of Systematic and Evolutionary Microbiology·Shih-Yi SheuWen-Ming Chen
Dec 18, 2007·Applied Microbiology and Biotechnology·Christiane DockGarabed Antranikian
May 2, 2009·International Journal of Systematic and Evolutionary Microbiology·A B ArunChiu-Chung Young
Jul 1, 2008·Applied Microbiology and Biotechnology·Shujun WangJianan Chen
Apr 20, 2010·International Journal of Systematic and Evolutionary Microbiology·Zhen-Shan DengGe-Hong Wei
Jul 25, 2009·International Journal of Systematic and Evolutionary Microbiology·Chiu-Chung YoungPeter Kämpfer
Mar 3, 2011·Biotechnology Progress·Monica C Santa-MariaBryon Sosinski
Nov 8, 2019·International Journal of Systematic and Evolutionary Microbiology·Kai LanBin Huang
Jul 1, 2020·The Journal of Microbiology·Han XueYong Li
Dec 13, 2006·International Journal of Systematic and Evolutionary Microbiology·Yi-Ju ChouWen-Ming Chen
Jun 7, 2007·International Journal of Systematic and Evolutionary Microbiology·Jui-Hsing ChouWen-Ming Chen
Mar 31, 2009·International Journal of Systematic and Evolutionary Microbiology·Mei-Chun LinWen-Ming Chen
Jul 27, 2010·International Journal of Systematic and Evolutionary Microbiology·P D RekhaFo-Ting Shen
Oct 10, 2008·International Journal of Systematic and Evolutionary Microbiology·Mei-Chun LinWen-Ming Chen
Apr 10, 2008·International Journal of Systematic and Evolutionary Microbiology·Jui-Hsing ChouWen-Ming Chen
Jul 17, 2012·International Journal of Systematic and Evolutionary Microbiology·P KämpferWen-Ming Chen
Mar 30, 2007·International Journal of Systematic and Evolutionary Microbiology·Jui-Hsing ChouChiu-Chung Young
Dec 27, 2011·International Journal of Systematic and Evolutionary Microbiology·Jing ZhangShun-Peng Li
May 3, 2007·International Journal of Systematic and Evolutionary Microbiology·Shih-Yi SheuWen-Ming Chen
Jul 13, 2007·International Journal of Systematic and Evolutionary Microbiology·Kuan-Yin LinWen-Ming Chen
Jan 12, 2010·International Journal of Systematic and Evolutionary Microbiology·Shih-Yi SheuWen-Ming Chen

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.