PMID: 8941763Apr 1, 1996Paper

Purification and properties of extracellular glucosyltransferase from Streptococcus bovis

Oral Microbiology and Immunology
Y UezonoH Mukasa

Abstract

Eight Streptococcus bovis strains were classified into 3 types on the basis of isoelectric point (pI) and molecular mass (M(r)) of extracellular glucosyltransferase. Strains ATCC 9809, 35034 and 43143 produced glucosyltransferase of pI 3.7 and M(r) 165 kDa; strains ATCC 15351, 27960 and 33317 produced glucosyltransferase of pI 4.1 and M(r) 140 kDa; strains ATCC 43085 and 43144 did not produce any glucosyltransferase. The glucosyltransferase form S. bovis 9809 was purified by Bio-Gel hydroxyapatite chromatography and DEAE-Toyopearl chromatography. The S. bovis 9809 glucosyltransferase was immunologically identical with the other 5 S. bovis glucosyltransferases and not related to mutants streptococcal glucosyltransferases. The specific activity, the optimum pH and the Km value for sucrose were 17.9 U/mg protein, 6.0 and 5.0 mM, respectively. The first 11 N-terminal amino acid residues of the glucosyltransferases were DETSAVTLTRE, and the region was hydrophilic. The glucosyltransferases from S. bovis 9809 and 3317 synthesized from sucrose 1, 6-alpha-D-glucan with 9 and 2 mol%, 1, 3, 6-alpha-branched glucose, respectively.

References

Aug 1, 1976·Journal of Medical Microbiology·M T Parker, L C Ball
Apr 1, 1975·Infection and Immunity·B TerleckyjG D Shockman
Mar 1, 1991·Journal of General Microbiology·R J Haisman, H F Jenkinson
Oct 15, 1985·Carbohydrate Research·D A Grahame, R M Mayer
Sep 1, 1987·Journal of Bacteriology·T ShirozaH K Kuramitsu
Sep 1, 1987·Journal of Bacteriology·J J FerrettiR R Russell
Jan 1, 1986·Medical Microbiology and Immunology·A R ThomsenM Volkert
Nov 1, 1974·Annals of Internal Medicine·W L Hoppes, P I Lerner
Dec 1, 1970·Archives of Oral Biology·J CarlssonS Wikner
Oct 1, 1971·Archives of Oral Biology·J Van HouteA J Pulkkinen
Aug 1, 1983·Journal of Medical Microbiology·I Crawford, C Russell
Mar 18, 1982·Biochimica Et Biophysica Acta·A ShimamuraH Mukasa
Apr 1, 1983·Journal of Clinical Microbiology·J G ReynoldsW M McCormack
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Feb 1, 1938·Journal of Bacteriology·J M Sherman

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