PMID: 7546557Sep 1, 1995Paper

Purification and properties of four monocot lectins from the family Araceae

Phytochemistry
S ShangaryR S Sandhu

Abstract

Four new monocot lectins from the tubers of araceous plants, namely, Arisaema consanguineum Schott (ACA), A. curvatum Kunth (ACmA) and Sauromatum guttatum Schott (SGA) from the tribe Areae, and Gonatanthus pumilus D. Don (GPA) from the tribe Colocasieae have been purified by affinity chromatography on asialofetuin-linked amino activated silica beads. These lectins possess similar physicochemical and biological properties. All the lectins gave a single peak on HPLC size exclusion and cation exchange columns, and a single band on PAGE, (pH 4.5). In SDS-PAGE, all the lectins gave a single band corresponding to a subunit of M(r) 1,3000. All the lectins yielded multiple peaks on anion-exchange column, multiple bands on non-denatured PAGE (pH 8.3) and a family of bands on isoelectric focusing. The lectins agglutinate rabbit, rat and sheep red blood cells (RBCs) but are inactive towards human ABO erythrocytes. The haemagglutination activity of these lectins is inhibited by asialofetuin only, while simple sugars/derivatives including chitin, porcine mucin and fetuin did not react. In serological studies against rabbit anti-SGA serum, all four lectins produced immunoprecipitin lines. The lectins within each tribe were identical but the ...Continue Reading

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Citations

Aug 30, 2008·Journal of Fluorescence·Poorva N DharkerSukhdev Singh Kamboj
Oct 17, 2012·Applied Biochemistry and Biotechnology·Thavamani Sureshkumar, Sulochana Priya
Sep 10, 2013·Applied Biochemistry and Biotechnology·Theolyta S AraújoBruno A M Rocha
Mar 25, 2005·Biochimica Et Biophysica Acta·Jagmohan Singh BainsA K Saxena
Jan 11, 2012·Bioscience, Biotechnology, and Biochemistry·Yuichiro NakagawaSuguru Oguri
May 19, 2004·Biochemical and Biophysical Research Communications·Jagmohan SinghSukhdev Singh Kamboj
Aug 8, 2006·Journal of Biochemistry and Molecular Biology·Manpreet KaurJatinder Singh
Dec 3, 2014·Biochimica Et Biophysica Acta·Patrícia R PereiraJoab T Silva
Jul 8, 2017·International Journal of Biological Macromolecules·Kshema ThakurManpreet Kaur

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