PMID: 64139Jan 1, 1976Paper

Purification and properties of human placenta arylsulphatase A

Archives internationales de physiologie et de biochimie
A A Farooqui

Abstract

1. Arylsulphatase A (arylsulphate sulphohydrolase E.N. 3.1.6.1) has been purified 7200-fold from human placenta using concanavalin A Sepharose chromatography. 2. Ultracentrifugation studies indicated that the purified enzyme was homogenous with respect to sedimentation coefficient and molecular weight and has a molecular weight of 102000. 3. The purified enzyme could hydrolyze cerebroside 3-sulphate, seminolipid and sulphogalactosylsphingosine under identical conditions. 4. The kinetic parameters for the hydrolysis of all sulphate esters used in the present study were the same. 5. Both seminolipid and sulphogalactosylsphingosine were competitive inhibitors for the hydrolysis of cerebroside-3-sulphate with an inhibition constant of 0.2 mM. 6. Kinetic parameters, metal ion effect and heat inactivation profile of enzyme suggest that the same active site of enzyme is responsible for the hydrolysis of cerebroside 3-sulphate, seminolipid and sulphogalactosylsphingosine.

References

Nov 5, 1974·Biochimica Et Biophysica Acta·A Jerfy, A B Roy
Nov 6, 1974·Biochemical and Biophysical Research Communications·A L FluhartyH Kihara
Mar 1, 1973·Journal of Neurochemistry·A A Farooqui, B K Bachawat
Nov 2, 1973·Biochimica Et Biophysica Acta·E R Graham, A B Roy
Jan 14, 1970·Biochimica Et Biophysica Acta·A B Roy
Feb 1, 1972·The Biochemical Journal·A A Farooqui, B K Bachhawat
Mar 25, 1968·Biochimica Et Biophysica Acta·E Mehl, H Jatzkewitz
Dec 1, 1963·Journal of Neurochemistry·J H AUSTINB K BACHHAWAT
Mar 1, 1964·Biochemistry·D A YPHANTIS
Jun 1, 1964·Journal of Biochemistry·L W NICHOL, A B ROY
Mar 1, 1965·Biochemistry·L W NICHOL, A B ROY

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Citations

Jan 1, 1977·Planta·A A FarooquiM M Afridi
Jan 1, 1984·Neurochemical Pathology·A A Farooqui, L A Horrocks
Jan 1, 1978·The International Journal of Biochemistry·A A Farooqui
Jan 31, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·A A Farooqui

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