PMID: 1250Nov 1, 1975

Purification and properties of isoenzymes of cinnamyl-alcohol dehydrogenase from soybean-cell-suspension cultures

European Journal of Biochemistry
D Wyrambik, H Grisebach

Abstract

Two isoenzymes of an NADP+ -dependent cinnamyl alcohol dehydrogenase and an NAD+ - dependent aliphatic alcohol dehydrogenase were extracted from cell suspension cultures of soybean (Glycine max L., var. Mandarin) which form lignin during growth. These enzymes could be separated from each other by chromatography on DEAE-cellulose and hydroxyapatite. The cinnamyl alcohol dehydrogenase isoenzymes were partially purified by (NH4)2SO4 fractionation, and column chromatography on DEAE-cellulose, Sephadex G-100, and hydroxyapatite. The molecular weight of the enzymes were estimated by the elution volumes from a Sephadex G-100 column and were found to be about 43,000 (isoenzyme 1) and 69,000 (isoenzyme 2). Maximum rates of reaction were observed in the case of coniferyl alcohol oxidation at pH 9.2 (Isoenzyme 1) and pH 8.8 (isoenzyme 2); in the reverse reaction pH 6.5 was optimal for isoenzyme 2. Whereas isoenzyme 1 is specific for coniferyl alcohol, isoenzyme 2 can also oxidize cinnamyl alcohol and a number of substituted cinnamyl alcohols, Km values for substituted cinnamaldehydes are 3-11 times lower than for the corresponding alcohols. Neither isoenzyme reacted with benzyl alcohol, anisic alcohol or ethanol. Substrate inhibition for ...Continue Reading

Citations

Dec 1, 1977·Veterinariia·A G Malakhov
Feb 23, 2002·Journal of Plant Physiology·M A BernardsN G Lewis
Oct 3, 2012·Journal of Experimental Botany·Christian BukhSøren K Rasmussen
Sep 8, 2011·Bioscience, Biotechnology, and Biochemistry·Motohiro NishimuraJulian Davies
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·I E SomssichK Hahlbrock
Nov 28, 2013·Journal of Applied Microbiology·Brahma N SinghHarikesh B Singh
Nov 5, 2013·TheScientificWorldJournal·Sudhamoy MandalPriyambada Acharya
Oct 7, 2006·Plant Physiology and Biochemistry : PPB·W D dos SantosO Ferrarese-Filho
Feb 23, 2011·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Tianjia JiangTiejin Ying
Nov 15, 2017·BMC Genomics·Weiying ZengXiangmin Tang
Jun 4, 2002·Nature Biotechnology·Gilles PilateC Halpin
May 5, 2005·Plant & Cell Physiology·Laigeng LiVincent L Chiang
Oct 19, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hye Lin ParkMan-Ho Cho
Jul 1, 2010·Comprehensive Reviews in Food Science and Food Safety·Rupinder SinghUpendra N Dwivedi

Related Concepts

Alcohol Oxidoreductases
Cinnamates
Drug Stability
Hydrogen-Ion Concentration
Alloenzymes
NADH
Glycine max
Structure-Activity Relationship

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