PMID: 6512859Dec 5, 1984

Purification and properties of native titin

Journal of Molecular Biology
J TrinickA Whiting


A procedure has been developed for the extraction and purification of the massive myofibrillar protein titin without exposing it to denaturing conditions. The form of the molecule that has been isolated is soluble at high ionic strength and alkaline pH, but precipitates in low salt or at pH values below 7. Sedimentation velocity experiments indicate that titin is a highly asymmetric molecule with a sedimentation coefficient of 13.4 S. This asymmetry is confirmed by electron microscopy of rotary-shadowed specimens, which shows string-like structures of diameter 40 A and lengths up to 8000 A. Significant differences were observed depending on whether the electron microscope specimens were prepared by spraying or by layering of the titin onto a mica substrate; we tentatively attribute these differences to elasticity in the titin, revealed by the high shearing forces that accompany spraying. In accord with this, the circular dichroism spectrum of titin indicates that its secondary structure is largely random coil, a conformation characteristic of elastic proteins such as elastin. Negative staining of titin again shows long string-like structures, but these can now be seen to have an appearance similar to a string of beads, where th...Continue Reading


Apr 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A B Fulton, W B Isaacs
Jul 9, 1999·Reviews of Physiology, Biochemistry and Pharmacology·R Horowits
Feb 15, 1988·Biochemical and Biophysical Research Communications·G P Kurzban, K Wang
Jan 1, 1996·Advances in Biophysics·B Bullard, K Leonard
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C AstierY Benyamin
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C MencarelliV Pallini
Jun 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J D FritzMarion L Greaser
Apr 1, 1985·Journal of Ultrastructure Research·A P MouldJ A Chapman
Feb 18, 2012·The New England Journal of Medicine·Daniel S HermanChristine E Seidman
Feb 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Maria E ZoghbiRoger Craig
Mar 1, 1986·The Journal of Cell Biology·C S Hill, K Weber
Aug 1, 1989·The Journal of Cell Biology·P Vibert, L Castellani
Nov 1, 1989·The Journal of Cell Biology·W B IsaacsA B Fulton
Sep 1, 1994·The Journal of Cell Biology·K J EilertsenT C Keller
Dec 1, 1995·The Journal of Cell Biology·Ahmed HoumeidaJ Trinick
Jan 12, 2013·Nucleic Acids Research·Shijun LiMarion L Greaser
Mar 26, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Larissa Tskhovrebova, J Trinick
Sep 15, 1992·European Journal of Biochemistry·J V SmallL E Thornell
Mar 1, 1997·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·G O SkeieN E Gilhus
Feb 6, 2013·Biochemistry. Biokhimii︠a︡·I M Vikhlyantsev, Z A Podlubnaya
Jul 16, 2013·Biochemistry. Biokhimii︠a︡·I M VikhlyantsevZ A Podlubnaya
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Larissa Tskhovrebova, J Trinick
Dec 13, 2000·Journal of Molecular and Cellular Cardiology·M M LeWinter
Feb 9, 2010·Journal of Molecular Biology·Larissa TskhovrebovaJ Trinick
Oct 1, 2008·Journal of Molecular Biology·Ahmed HoumeidaJ Trinick
Nov 23, 2011·Journal of Cellular Biochemistry·Shijun LiMarion L Greaser
Feb 1, 1991·Current Opinion in Cell Biology·J Trinick
Aug 10, 2004·Biophysical Journal·Mark C LeakeRobert M Simmons

Related Concepts

ELN gene
Anatomical Layer
Chromatography, DEAE-Cellulose
TTN gene
Electron Microscopy, Diagnostic
Coils Program
Electron Microscopy
Protein KINASE

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.