PMID: 9557875Apr 29, 1998Paper

Purification and properties of recombinant beta-galactosidase from Bacillus circulans

Glycoconjugate Journal
H FujimotoK Ajisaka

Abstract

A gene encoding beta-galactosidase from Bacillus circulans which had hydrolysis specificity for the beta1-3 linkage was expressed in Escherichia coli. The beta-galactosidase was purified from crude cell lysates of E. coli by column chromatographies on Resource Q and Sephacryl S-200 HR. The enzyme released galactose with high selectivity from oligosaccharides which had terminal beta1-3 linked galactose residues. However it did not hydrolyse beta1-4 linked galactooligosaccharides. Moreover, Galbeta1-3GlcNAc, Galbeta1-3GalNAc, and their p-nitrophenyl glycosides were regioselectively synthesized in 10-46% yield by the transglycosylation reaction using this enzyme.

Citations

Oct 27, 2004·Bioscience, Biotechnology, and Biochemistry·Mariko Miyasato, Katsumi Ajisaka
Jun 15, 2011·Bioscience, Biotechnology, and Biochemistry·Jingyuan SongKazuhiro Nakanishi
Nov 28, 2009·Applied Microbiology and Biotechnology·Ah-Reum Park, Deok-Kun Oh
Mar 16, 2013·World Journal of Microbiology & Biotechnology·Guo-Xiang WangBing-Hua Jiao
Sep 21, 2010·Applied Microbiology and Biotechnology·Onladda JuajunMontarop Yamabhai
Feb 11, 2011·Bioscience, Biotechnology, and Biochemistry·Jingyuan SongKazuhiro Nakanishi
Aug 17, 2004·The Journal of Biological Chemistry·Masaki KurogochiYuan Chuan Lee
Oct 26, 1999·Biochemical and Biophysical Research Communications·H IwaseK Hotta
Jul 30, 1999·Biochemical and Biophysical Research Communications·H IwaseK Hotta
Jul 20, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ikuko NakamuraYutaka Kobayashi
Oct 20, 2004·Journal of Molecular Biology·A L RojasI Polikarpov
Jun 9, 2012·Journal of Agricultural and Food Chemistry·Barbara Rodriguez-ColinasFrancisco J Plou
Jun 30, 2015·Chemical Reviews·Angeles FarránRobert J Linhardt

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