PMID: 6983681Oct 25, 1982Paper

Purification and properties of the Hpa I methylase

Nucleic Acids Research
O J YooK L Agarwal

Abstract

The purification and catalytic properties of the homogeneous Hpa I methylase is described. The enzyme exists as a single polypeptide chain with a molecular weight of 37,000 +/- 2,000 was shown by sedimentation equilibrium and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The Hpa I methylase transfers methyl groups of S-adenosylmethionine to adenine present in the recognition sequence d(G-T-T-A-A*-C), A* is the N6 methyl adenosine. An average of 2.1 methyl groups per recognition site are transferred by the Hpa I methylase.

Citations

Feb 27, 1996·Cancer Letters·G Ramesh, U N Das
Jan 1, 1985·Nucleic Acids Research·M McClelland, M Nelson
Feb 25, 1992·Nucleic Acids Research·H ItoI Kato
Feb 9, 2006·Nucleic Acids Research·Chad B Thomas, Richard I Gumport
Mar 1, 1987·Journal of Bacteriology·M EhrlichC W Gehrke

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