Purification and properties of the voltage-dependent anion channel of the outer mitochondrial membrane.

Journal of Bioenergetics and Biomembranes
F Palmieri, V De Pinto

Abstract

The methods for the purification of functionally active mitochondrial porin or voltage-dependent anion channel of the outer mitochondrial membrane are critically evaluated. Two rapid and efficient methods are now available. Both make use of a hydroxyapatite/celite column as a single chromatographic step. However, in one method with long polar head-group detergents, porin passes through the column, whereas in the other method, with shorter polar head-group detergents, porin is first bound to the column and then eluted by the addition of salts. On the basis of these results, a model for the arrangement of porin in the detergent-protein micelles is proposed.

References

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Citations

Mar 28, 2003·Biochemical and Biophysical Research Communications·Yong ShiHong Tang
Jul 6, 2012·The Journal of Biological Chemistry·Tatiana K RostovtsevaSergey M Bezrukov
Feb 1, 1992·Journal of Bioenergetics and Biomembranes·V D De Pinto, F Palmieri
Dec 3, 2014·Biochimica Et Biophysica Acta·Debanjan TewariAmal Kanti Bera
Dec 3, 2014·FEBS Letters·Brian P WeiserRoderic G Eckenhoff
Mar 30, 2010·Molecular Aspects of Medicine·Varda Shoshan-BarmatzNir Arbel
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·M C Sorgato, O Moran
Jul 25, 2012·Biophysical Journal·Philip A GurnevSergey M Bezrukov
May 9, 2006·Biochimica Et Biophysica Acta·Inge RomanMartin Zizi
May 20, 2018·The Journal of Biological Chemistry·Tatiana K RostovtsevaSergey M Bezrukov
Apr 3, 2014·Physiological Reviews·Ildiko Szabo, Mario Zoratti
Jun 26, 2014·The Journal of Biological Chemistry·Oscar TeijidoSergey M Bezrukov
Jan 15, 2020·The Journal of General Physiology·María Queralt-MartínTatiana K Rostovtseva
Jan 10, 2017·Frontiers in Oncology·Simona ReinaVito De Pinto
Nov 25, 2019·Cellular and Molecular Life Sciences : CMLS·Amandine RoviniTatiana K Rostovtseva
Apr 24, 2018·Frontiers in Chemistry·Andrea MagrìVito De Pinto

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