PMID: 2123854Dec 1, 1990Paper

Purification and properties of thermostable xylanase and beta-xylosidase produced by a newly isolated Bacillus stearothermophilus strain

Journal of Bacteriology
T NanmoriY Kawamura

Abstract

We isolated a thermophilic bacterium that produces both xylanase and beta-xylosidase. Based on taxonomical research, this bacterium was identified as Bacillus stearothermophilus. Each extracellular enzyme was separated by hydrophobic chromatography by using a Toyopearl HW-65 column, followed by gel filtration with a Sephacryl S-200 column. Each enzyme in the culture was further purified to homogeneity (62-fold for xylanase and 72-fold for beta-xylosidase) by using a fast protein liquid chromatography system with a Mono Q HR 5/5 column. The optimum temperatures were 60 degrees C for xylanase and 70 degrees C for beta-xylosidase. The isoelectric points and molecular masses were 5.1 and 39.5 kDa for xylanase and 4.2 and 150 kDa for beta-xylosidase, respectively. Heat treatment at 60 degrees C for 1 h did not cause inhibition of the activities of these enzymes. The action of the two enzymes on xylan gave only xylose.

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Citations

Jan 16, 2014·Applied Biochemistry and Biotechnology·Nawel BoucherbaSaid Benallaoua
Jan 1, 1993·FEMS Microbiology Reviews·J A Thomson
Jan 1, 1997·Critical Reviews in Biotechnology·A Sunna, G Antranikian
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Nov 2, 2012·Applied Biochemistry and Biotechnology·Jaya Ram SimkhadaJin Cheol Yoo
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