Purification and properties of xanthine dehydrogenase from Pseudomonas acidovorans.

Biochimica Et Biophysica Acta
I L Sin

Abstract

Xanthine dehydrogenase (EC 1.2.1.37) from Pseudomonas acidovorans has been purified to near homogeneity (approx. 65-fold). The enzyme has a molecular weight of about 275 000. Electrophoresis in gels containing sodium dodecyl sulphate showed the presence of two types of subunit with molecular weights of about 81 000 and 63 000. Thus the intact molecule probably contains two of each type of subunit. Xanthine and hypoxanthine are good substrates, and NAD+ is an effective electron acceptor. With xanthine and NAD+ as substrates the purified enzyme has a specific activity of about 20 mumol NADH formed/min per mg protein. Michaelis constants for xanthine and NAD+ are 0.07 and 0.12 mM, respectively, and for hypoxanthine and NAD+ 0.29 and 0.16 mM, respectively.

References

May 21, 1974·Biochemistry·C TanfordS Makino
May 1, 1974·Analytical Biochemistry·R K Scopes
Jul 1, 1968·Archives of Biochemistry and Biophysics·J L Hedrick, A J Smith
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

❮ Previous
Next ❯

Citations

Sep 30, 2006·Biotechnology Letters·Swati Sucharita Dash, Sathyanarayana N Gummadi
Aug 30, 2011·Pakistan Journal of Biological Sciences : PJBS·K Khucharoenphaisan, K Sinma
Feb 5, 2014·International Journal of Molecular Sciences·Ana Rita Otrelo-CardosoTeresa Santos-Silva
Sep 24, 1999·Biochemical and Biophysical Research Communications·K M MadyasthaY S Madhavi
Aug 19, 2016·Bioengineered·Cheng-Hua WangXin-Hui Xing
Oct 1, 1979·Biotechnology and Bioengineering·J TramperH C van der Plas
Jun 1, 1976·Bacteriological Reviews·G D Vogels, C Van der Drift
Aug 1, 1978·Journal of Bacteriology·C A Woolfolk, J S Downard
Sep 1, 1979·Microbiological Reviews·D C Yoch, R P Carithers
Jun 1, 1977·Journal of Bacteriology·C A Woolfolk, J S Downard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.