PMID: 8585087Sep 1, 1995Paper

Purification and sequence determination of a new muscarinic toxin (MT4) from the venom of the green mamba (Dendroaspis angusticeps)

Toxicon : Official Journal of the International Society on Toxinology
A VandermeersE Karlsson

Abstract

A toxin which partially inhibited [3H]N-methylscopolamine binding to rat brain muscarinic receptors was purified from the venom of green mamba, Dendroaspis angusticeps. The N-terminal sequence (up to 45 amino acids) was determined by automated Edman degradation of the whole molecule. The complete sequence was elucidated after enzymatic cleavage with endoproteinase Arg-C or endoproteinase Lys-C and peptide fragments purification. The identity of the C-terminal amino acid was confirmed by hydrazinolysis. The new toxin (MT4) had eight half-cystines and 66 amino acids. It differed from muscarinic toxin MT1 by a single substitution in position 57 (arginine in MT1, histidine in MT4), proximal to the sixth half-cystine.

References

Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·J L Middlebrook
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J R de WeilleM Lazdunski
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·F DucancelJ C Boulain
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·E KarlssonA Adem
Jan 1, 1990·Annual Review of Pharmacology and Toxicology·E C HulmeN J Buckley
Apr 1, 1989·Trends in Neurosciences·T I Bonner
Jan 1, 1988·Journal of Receptor Research·M WaelbroeckJ Christophe
Jan 1, 1985·Pharmacology & Therapeutics·E KarlssonD Rodríguez-Ithurralde
Jun 1, 1993·Pharmacology & Therapeutics·M P Caulfield
Apr 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·M JolkkonenE Karlsson
Jan 1, 1993·Trends in Pharmacological Sciences·M F HibertA Bruinvels
Mar 9, 1994·Annals of the New York Academy of Sciences·E KarlssonC Wernstedt

❮ Previous
Next ❯

Citations

May 9, 2000·Biochemical and Biophysical Research Communications·J NäsmanK E Akerman
Jun 3, 1996·European Journal of Pharmacology·M WaelbroeckA Vandermeers
Mar 23, 2004·European Journal of Pharmacology·Maria C OlianasPierluigi Onali
Jan 1, 1997·Life Sciences·A Adem, E Karlsson
Mar 1, 2000·Toxicon : Official Journal of the International Society on Toxinology·D JerusalinskyC Cerveñansky
Mar 18, 2000·Pharmacology & Therapeutics·K N Bradley
Dec 1, 1995·European Journal of Biochemistry·M JolkkonenE Karlsson
Apr 13, 2011·Acta Physiologica·K Näreoja, J Näsman
Aug 2, 2006·Autonomic & Autacoid Pharmacology·A M HamrouniK J Broadley
May 12, 2011·British Journal of Pharmacology·K NäreojaJ Näsman
Feb 1, 2005·Life Sciences·Pierluigi OnaliMaria C Olianas
Sep 29, 2004·The Journal of Biological Chemistry·Anu KukkonenJohnny Näsman
Dec 13, 2007·The Journal of Biological Chemistry·Alex I ChernyavskySergei A Grando
Nov 18, 2000·European Journal of Biochemistry·V V KukhtinaY N Utkin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.