Purification and some physicochemical properties of staphylococcal enterotoxin D

H C Chang, M S Bergdoll


A method was developed for the isolation of staphylococcal enterotoxin D in highly purified form from cultures of Staphylococcus aureus strain 1151m. The method involves removal of the toxin from the culture supernatant fluid with the ion-exchange resin CG-50 followed by chromatography on carboxymethylcellulose (twice) and by gel filtration on Sephadex G-75 (twice). The purified toxin is homogeneous by polyacrylamide gel and sodium dodecyl sulfate-polyacrylamide gel electrophoresis and double gel diffusion tests. It is a simple, colorless, antigenic protein with an isoelectric point of 7.4 as determined by isoelectric focusing. Its molecular weight was determined to be 27 300 +/- 700 by molecular sieve chromatography on Sephadex G-100 and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its serological activity is stable over a wide range of pH values (1.2--10.7). The enterotoxin consists of 236 amino acid residues and contains no free sulfhydryl groups. End-group analysis showed serine to be the NH2-terminal amino acid and lysine to be the COOH-terminal amino acid.


Jul 23, 1965·Annals of the New York Academy of Sciences·E P Casman
Nov 1, 1972·Japanese Journal of Microbiology·H Igarashi
Sep 7, 1967·Biochemical and Biophysical Research Communications·A L ShapiroJ V Maizel
Jun 1, 1965·Biochemistry·E J SchantzM S Bergdoll
Oct 1, 1966·Biochemistry·F S ChuM S Bergdoll
Oct 23, 1970·Biochemical and Biophysical Research Communications·M Okude, S Iwanaga
Nov 1, 1959·Archives of Biochemistry and Biophysics·M S BergdollG M DACK
Aug 1, 1965·Analytical Chemistry·J V BENSON, J A PATTERSON
Jan 1, 1945·The Biochemical Journal·F Sanger


Jan 1, 1982·Pharmacology & Therapeutics·J H Freer, J P Arbuthnott
Jul 2, 1999·Letters in Applied Microbiology·A MeyrandC Vernozy-Rozand
Jan 1, 1990·Society for Applied Bacteriology Symposium Series·H S Tranter, R D Brehm
Jul 1, 2010·Toxins·Sanjeev KumarAnthony T Vella
Aug 1, 2010·Toxins·Irina V PinchukVictor E Reyes
Mar 15, 1982·Biochemical and Biophysical Research Communications·G N Stelma, M S Bergdoll
Nov 19, 2011·FEMS Microbiology Reviews·Jacques-Antoine HennekinneSylviane Dragacci
Aug 1, 1984·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·C YangO J Hübschle
Jun 1, 1991·FEMS Microbiology Immunology·A C WoodJ P Arbuthnott

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