Dec 18, 1975

Purification and some properties of a novel maltohexaose-producing exo-amylase from Aerobacter aerogenes

Biochimica Et Biophysica Acta
K KainumaS Suzuki

Abstract

Maltohexaose producing amylase (EC 3.2.1.-) is the fourth known exo-amylase, the three previously known being glucoamylase, beta-amylase and Pseudomonas stutzeri maltotetraose producing amylase. The enzyme after release from Aerobacter aerogenes cells by 0.1% sodium lauryl sulfate extraction was purified by ammonium sulfate precipitation, DEAE-Sephadex column chromatography and Sephadex G-100 gel filtration to 80-fold of the original sodium lauryl sulfate extract activity, It gave a single band on disc electrophoresis, and the molecular weight by gel filtration was 54 000. This amylase showed maximal activity at 50 degrees C and pH 6.80. The pH stability range was relatively wide, the enzyme retaining more than 90% of its initial activity in the range of 6.50-9.0. 80% of the activity was retained after 15 min at 50 degrees C. This enzyme produced maltohexaose from starch, amylose and amylopectin by exo-attack, but did not act on alpha- or beta-cyclodextrin, pullulan or maltohexaitol. Also the enzyme acted on beta-limit dextrins of amylopectin and glycogen to form branched oligosaccharides. The unusual reaction of this enzyme on beta-limit dextrin is discussed from the standpoint of the stereochemistry of 1,4-alpha- and 1,6-alph...Continue Reading

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Mentioned in this Paper

Glucan 1,4-alpha-Glucosidase
Cations, Divalent
Calcium
Dextrin
Structure-Activity Relationship
Betadex
Pseudo brand of pseudoephedrine
maltohexaitol
Enterobacter aerogenes bacterium
Pseudomonas stutzeri

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