PMID: 1094Dec 18, 1975

Purification and some properties of a novel maltohexaose-producing exo-amylase from Aerobacter aerogenes

Biochimica Et Biophysica Acta
K KainumaS Suzuki

Abstract

Maltohexaose producing amylase (EC 3.2.1.-) is the fourth known exo-amylase, the three previously known being glucoamylase, beta-amylase and Pseudomonas stutzeri maltotetraose producing amylase. The enzyme after release from Aerobacter aerogenes cells by 0.1% sodium lauryl sulfate extraction was purified by ammonium sulfate precipitation, DEAE-Sephadex column chromatography and Sephadex G-100 gel filtration to 80-fold of the original sodium lauryl sulfate extract activity, It gave a single band on disc electrophoresis, and the molecular weight by gel filtration was 54 000. This amylase showed maximal activity at 50 degrees C and pH 6.80. The pH stability range was relatively wide, the enzyme retaining more than 90% of its initial activity in the range of 6.50-9.0. 80% of the activity was retained after 15 min at 50 degrees C. This enzyme produced maltohexaose from starch, amylose and amylopectin by exo-attack, but did not act on alpha- or beta-cyclodextrin, pullulan or maltohexaitol. Also the enzyme acted on beta-limit dextrins of amylopectin and glycogen to form branched oligosaccharides. The unusual reaction of this enzyme on beta-limit dextrin is discussed from the standpoint of the stereochemistry of 1,4-alpha- and 1,6-alph...Continue Reading

Citations

Jan 11, 2007·Journal of Industrial Microbiology & Biotechnology·Chao-Hsun Yang, Wen-Hsiung Liu
Apr 1, 1983·Biotechnology and Bioengineering·T NakakukiK Kainuma
Jan 1, 1989·Critical Reviews in Biochemistry and Molecular Biology·M Vihinen, P Mäntsälä
Sep 5, 2020·Journal of Food Science·Ryota HosomiKenji Fukunaga

Related Concepts

Amylases
Amylopectin
Calcium
Cations, Divalent
Chloromercuribenzoates
Dextrins
Enterobacter
Paracolobactrum
Glycogen
Hydrogen-Ion Concentration

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