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Purification and some properties of alkaline pullulanase from a strain of bacillus no. 202-1, an alkalophilic microorganism

Biochimica Et Biophysica Acta

Jul 27, 1975

N NakamuraK Horikoshi

PMID: 238632

Abstract

Pullulanase (pullulan 6-glucanohydrolase EC 3.2.1.41) was purified about 290-fold from the culture fluid of Bacillus No. 202-1 by DEAE-cellulose adsorption, acetone fractionation, (NH4) 2SO4 precipitation and DEAE--cellulose column chromatography followed by Sephadex G-200 molecular sie...read more

Mentioned in this Paper

Mercury
SDS-PAGE
Copper
Amylopectin
Zinc
Exoglycosidases
Glycogen
Protein Denaturation
Hydrogen-Ion Concentration
Magnesium
Paper Details
References
  • References2
  • Citations11
12

Purification and some properties of alkaline pullulanase from a strain of bacillus no. 202-1, an alkalophilic microorganism

Biochimica Et Biophysica Acta

Jul 27, 1975

N NakamuraK Horikoshi

PMID: 238632

DOI:

Abstract

Pullulanase (pullulan 6-glucanohydrolase EC 3.2.1.41) was purified about 290-fold from the culture fluid of Bacillus No. 202-1 by DEAE-cellulose adsorption, acetone fractionation, (NH4) 2SO4 precipitation and DEAE--cellulose column chromatography followed by Sephadex G-200 molecular sie...read more

Mentioned in this Paper

Mercury
SDS-PAGE
Copper
Amylopectin
Zinc
Exoglycosidases
Glycogen
Protein Denaturation
Hydrogen-Ion Concentration
Magnesium

Related Papers

Paper Details
References
  • References2
  • Citations11
12
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