Purification and stability of octameric mitochondrial creatine kinase isoform from herring (Clupea harengus) organ of vision

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Natalia NiedźwieckaEdward F Skorkowski

Abstract

Creatine kinases (CKs) constitute a large family of isoenzymes that are involved in intracellular energy homeostasis. In cells with high and fluctuating energy requirements ATP level is maintained via phosphocreatine hydrolysis catalyzed by creatine kinase. In contrast to invertebrates and higher vertebrates, in poikilothermic vertebrates the adaptations for the regulation of energy metabolism by changes in the oligomeric state of CK isoforms are not well known. The present study aimed at identification of herring eye CK isoforms and focuses on factors affecting the CK-octamer stability. In addition to the CK octamer, three different dimeric isoforms of CK were detected by cellulose acetate native electrophoresis. Destabilization of octamer was studied in the presence of TSAC substrates and about 50% of octamers dissociated into dimers within 24h. Moreover, we found that the increase of temperature from 4 °C to 30 °C caused rapid inactivation of dimers in TSAC-treated samples but did not affect octameric structures. In a thermostability assay we demonstrated that octamers retain their activity even at 50 °C. Our results indicate that destabilization of the octameric structure can lead to loss of enzyme activity at higher temper...Continue Reading

References

Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T WallimannH M Eppenberger
Apr 1, 1994·Molecular and Cellular Biochemistry·S M MühlebachM Wyss
Aug 1, 1996·Molecular Reproduction and Development·C SaudraisM Loir
Apr 16, 1998·FEBS Letters·W R EllingtonA O Pineda
Apr 5, 2000·The Journal of Biological Chemistry·U Schlattner, T Wallimann
Aug 12, 2000·Neuron·S HaverkampH Wässle
Jan 22, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·G Harder, R McGowan
May 20, 1971·FEBS Letters·H M. EppenbergerH Ursprung
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Li-Yih LinChing-Feng Weng
Apr 3, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Paul WinnardMichael E Vayda
Mar 15, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Katarzyna Grzyb, Edward F Skorkowski
Jun 3, 2005·American Journal of Physiology. Cell Physiology·Monica L AcostaDavid L Christie
Mar 28, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Katarzyna Grzyb, Edward F Skorkowski
Mar 3, 2007·Gene·Matt BertinW Ross Ellington
Apr 13, 2010·International Journal of Biological Macromolecules·Yan-Song GaoHai-Meng Zhou
Jun 19, 2010·International Journal of Biological Macromolecules·Yan-Song GaoHai-Meng Zhou
Mar 31, 2011·Amino Acids·Theo WallimannUwe Schlattner
Dec 25, 2012·BMC Evolutionary Biology·Amber Gf TeacherChristopher W Wheat
Aug 26, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·K M O'BrienM Ortego

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Citations

Mar 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Janusz J PetkowskiSara Seager

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