PMID: 9449776Jan 1, 1997Paper

Purification and the effect of manganese ions on the activity of carboxymethylcellulases from Aspergillus niger and Cellulomonas biazotea

Folia Microbiologica
K S SiddiquiM I Rajoka

Abstract

Carboxymethylcellulases (CMCases) from Aspergillus niger and Cellulomonas biazotea were purified by a combination of ammonium sulfate precipitation, anion-exchange and gel-filtration chromatography with a 12- and 9-fold increase in the purification factor. The native and subunit molar mass of CMCase from A. niger were 40 and 25-57 kDa, respectively, while those from C. biazotea were 23 and 20-30 kDa, respectively. Low concentrations of Mn2+ activated the enzymes from both organisms (mixed activation) with apparent activation constants of 0.80 and 0.45 mmol/L of CMCases from A. niger and C. biazotea, respectively, while at higher CMC concentrations Mn2+ inhibited the enzymes (mixed and partial uncompetitive inhibition). The reason for this complex behavior is that more than one Mn2+ bind to the same enzyme form with the apparent average inhibition constants of 2.7 and 1.3 mmol/L for CMCases from A. niger and C. biazotea, respectively.

References

Apr 1, 1992·The Biochemical Journal·M Rangarajan, B S Hartley
Jan 1, 1990·Methods in Enzymology·C R Merril
Jan 1, 1990·Methods in Enzymology·E F Rossomando
Jan 1, 1990·Methods in Enzymology·E Stellwagen
Jan 19, 1989·Biochimica Et Biophysica Acta·W A Geary, S L Gonias
Feb 1, 1988·Applied and Environmental Microbiology·D Lupo, F Stutzenberger

❮ Previous
Next ❯

Citations

Jan 30, 2008·Journal of Industrial Microbiology & Biotechnology·Abdul JabbarMuhammad Aslam Malana
Apr 9, 2013·Folia Microbiologica·Jean Carlos Rodrigues SilvaJoão Atilio Jorge
Nov 12, 2005·The Journal of Medicine and Philosophy·Albert W Musschenga
Dec 19, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Sarita Keni, Narayan S Punekar
Nov 19, 2017·Applied Biochemistry and Biotechnology·Luana Parras MeleiroRosa Prazeres Melo Furriel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.