Purification and tissue distribution of rat cathepsin L

Journal of Biochemistry
Y BandoN Katunuma

Abstract

Cathepsin L was purified to apparent homogeneity from rat kidney. The molecular weight of the enzyme was estimated to be 30,000, but part of the enzyme was found to consist of two polypeptide chains of Mr 25,000 and 5,000. Antibody against rat kidney cathepsin L did not cross-react with rat cathepsin B or H and detected only cathepsin L in crude rat tissue preparations on immunoblotted sheets. The concentrations of cathepsin L in various rat tissues and peripheral blood cells of rats were determined by a sensitive immunoassay, in which the minimum detectable amount of cathepsin L was 20 pg/assay. The concentration of cathepsin L was found to be highest in the kidneys, where it was more than 3 times higher than in the liver, spleen, lungs, and brain. Nervous tissues, especially the cerebellar cortex, also contained fairly high concentrations of cathepsin L, but the heart, skeletal muscle, and gastrointestinal tract contained low concentrations, as did peripheral blood cells. The cathepsin L content of macrophages was 20% of that of cathepsin B. The concentrations of cathepsin L in lymphocytes, neutrophils, and erythrocytes were 10%, 20%, and less than 0.2%, respectively, of those in resident macrophages.

Citations

Oct 19, 1987·FEBS Letters·K IshidohK Suzuki
Oct 3, 1994·FEBS Letters·K Ishidoh, E Kominami
Jan 1, 1994·Acta Neuropathologica·T KumamotoM Ando
Apr 17, 2012·Clinical Reviews in Bone and Mineral Metabolism·Sara Sjöberg, Guo-Ping Shi
Feb 11, 2014·The Protein Journal·Azharul IslamEikichi Hashimoto
Aug 17, 1987·European Journal of Biochemistry·K WadaT Tanabe
Apr 1, 1991·DNA and Cell Biology·F QianD F Steiner
Sep 30, 1991·International Journal of Cancer. Journal International Du Cancer·S ShujaM J Murnane
Jan 1, 1990·Journal of Enzyme Inhibition·T SasakiT Murachi
Aug 15, 1988·FEBS Letters·T Towatari, N Katunuma
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W RothC Peters
May 20, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·S KudoK Kawano
Oct 24, 2002·The Journal of Dermatology·Piti PalungwachiraHideoki Ogawa
Jun 27, 2008·American Journal of Physiology. Renal Physiology·Wayne D ComperLeileata M Russo
Apr 30, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shinsuke KataokaKenji Yamamoto
Jun 20, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Masayuki SuyamaYasuo Uchiyama
Jan 1, 1989·Preparative Biochemistry·R Pike, C Dennison
Nov 1, 1986·Bioscience Reports·D BéchetC Deval
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shunichi Fukuda, Geert W Schmid-Schönbein
Nov 8, 2008·Current Diabetes Reports·Wayne D Comper, Leileata M Russo
Apr 17, 2004·Pathology International·Yasuko YoshiokaYoshinosuke Fukuchi
Mar 3, 2009·Biological & Pharmaceutical Bulletin·Katsuyuki TakahashiEiki Kominami
Nov 25, 2017·BMC Research Notes·Chihiro Tohda, Michihisa Tohda
Sep 14, 2004·Molecular Pharmacology·Tomoko KadowakiKenji Yamamoto
Aug 7, 2009·Journal of Cellular and Molecular Medicine·Angèle ChopardBernard J Jasmin
Oct 13, 2016·The Journal of Biological Chemistry·Chang LiuFang Li
Dec 1, 1991·Molecular Reproduction and Development·R T HamiltonM Nilsen-Hamilton
Mar 1, 1997·JPEN. Journal of Parenteral and Enteral Nutrition·M KishibuchiM Monden
Apr 1, 1994·In Vitro Cellular & Developmental Biology. Animal·N Brière, P Chailler

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