PMID: 3863754Nov 4, 1985Paper

Purification and tissue-specific expression of casein kinase from the lactating guinea-pig mammary gland

European Journal of Biochemistry
A MooreR K Craig

Abstract

A serine-specific casein kinase, an integral membrane protein of the lactating guinea-pig mammary gland, has been purified from a Golgi-enriched membrane fraction, using a combination of sucrose gradient centrifugation and chromatography on ATP-agarose. The enzyme comprises a polypeptide of estimated Mr 74 000 as judged by sodium dodecyl sulphate/polyacrylamide gel electrophoresis, compared with a monomer Mr of 50 000 as determined by sucrose gradient centrifugation in the presence of 500 mM NaCl and 0.1% Triton X-100. Kinetic studies show that the purified enzyme exhibits kinetic constants distinctly different from the rabbit reticulocyte casein kinases I and II, whilst polyclonal antisera raised against the mammary gland enzyme did not cross-react with soluble liver or reticulocyte protein kinase activities. Immunoblotting and immunocytochemical analyses demonstrate the mammary gland enzyme's apparently unique location in lactating mammary gland tissue. Comparative studies with polyclonal antisera raised against bovine galactosyltransferase, show that casein kinase and galactosyltransferase have a similar intracellular localisation in the lactating mammary gland as judged by immunocytochemistry at the light level, but that ca...Continue Reading

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Citations

Aug 19, 2000·The Biochemical Journal·J S DuncanR D Burgoyne
Dec 30, 1987·European Journal of Biochemistry·S M Foord, R K Craig
Nov 14, 2015·Expert Opinion on Therapeutic Targets·Giorgio Cozza, Lorenzo A Pinna
Jan 2, 2013·Trends in Biochemical Sciences·Vincent S TagliabracciJack E Dixon
Sep 10, 2014·Journal of Dairy Science·E BijlH Bovenhuis
Jul 19, 2013·Biochemical Society Transactions·Vincent S TagliabracciJack E Dixon
May 16, 2014·The Biochemical Journal·Andrea VenerandoLorenzo A Pinna
Dec 22, 1989·European Journal of Biochemistry·F MeggioL A Pinna
Dec 18, 1989·FEBS Letters·P AgostinisW Merlevede
Mar 25, 2021·The Journal of Biological Chemistry·Carolyn A WorbySourav Banerjee
May 2, 1998·Biochimica Et Biophysica Acta·N D RidgwayH W Cook
Sep 24, 1990·Biochimica Et Biophysica Acta·L A Pinna
Jul 21, 1989·Biochimica Et Biophysica Acta·T D Friedrich, V M Ingram

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