Purification, biochemical, and structural characterization of a novel fibrinolytic enzyme from Mucor subtilissimus UCP 1262

Bioprocess and Biosystems Engineering
Thiago Pajeú NascimentoAttilio Converti

Abstract

Fibrinolytic proteases are enzymes that degrade fibrin. They provide a promising alternative to existing drugs for thrombolytic therapy. A protease isolated from the filamentous fungus Mucor subtilissimus UCP 1262 was purified in three steps by ammonium sulfate fractionation, ion exchange, and molecular exclusion chromatographies, and characterized biochemically and structurally. The purified protease exhibited a molecular mass of 20 kDa, an apparent isoelectric point of 4.94 and a secondary structure composed mainly of α-helices. Selectivity for N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide as substrate suggests that this enzyme is a chymotrypsin-like serine protease, whose activity was enhanced by the addition of Cu2+, Mg2+, and Fe2+. The enzyme showed a fibrinolytic activity of 22.53 U/mL at 40 °C and its contact with polyethylene glycol did not lead to any significant alteration of its secondary structure. This protein represents an important example of a novel fibrinolytic enzyme with potential use in the treatment of thromboembolic disorders such as strokes, pulmonary emboli, and deep vein thrombosis.

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Oct 1, 1973·Photochemistry and Photobiology·E A BursteinM N Ivkova
Oct 1, 1993·Bioscience, Biotechnology, and Biochemistry·N NakajimaH Sumi
Sep 29, 1998·Analytical Biochemistry·S H KimW Y Lee
Oct 12, 2000·Toxicon : Official Journal of the International Society on Toxinology·Y S KohD S Kim
Oct 1, 1952·Archives of Biochemistry and Biophysics·T ASTRUP, S MULLERTZ
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Weon-Kyoo YouKwang-Hoe Chung
Aug 5, 2008·CNS & Neurological Disorders Drug Targets·Xiaoying WangEng H Lo
Aug 11, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yoshiko UesugiYasuhiko Tabata
Dec 4, 2010·Applied Microbiology and Biotechnology·Sirma YeginCanan Tari
Jul 1, 2011·The Journal of Microbiology·Won Sik YeoYong Kee Jeong
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Xiuyun JuJihong Jiang
Feb 9, 2013·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xu ZhangFei Tang
Sep 6, 2013·International Journal of Biological Macromolecules·Shihai HuangZhiqun Liang
Oct 12, 2013·Applied Biochemistry and Biotechnology·Chittoor Jabeena Thaz, Gurunathan Jayaraman
Aug 21, 2014·Nature Communications·Rafi ChapanianJayachandran N Kizhakkedathu
Jan 2, 2016·International Journal of Biological Macromolecules·Zhibin SunZhongli Cui
May 18, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Thiago Pajeú NascimentoAttilio Converti

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Citations

Nov 18, 2020·International Journal of Biological Macromolecules·Jihen ElleuchSlim Abdelkafi
Jun 5, 2021·International Journal of Biological Macromolecules·Jéssica Miranda do NascimentoAna Cristina Lima Leite
Sep 2, 2018·International Journal of Biological Macromolecules·Osmar Soares da SilvaTatiana Souza Porto

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