PMID: 2504286Jun 13, 1989Paper

Purification, characterization, and amino acid sequence of the mating pheromone Er-10 of the ciliate Euplotes raikovi

Biochemistry
S RaffioniR A Bradshaw

Abstract

The mating pheromone Er-10 from mat-10 homozygous Euplotes raikovi was purified by a three-step purification procedure with an overall yield of 62%. It was identified as a protein of molecular weight 8000 having an isoelectic point of 3.9. Its complete primary structure was determined by automated Edman degradation of the whole protein after performic acid oxidation and of peptides generated by cyanogen bromide and Staphylococcus aureus V8 protease. The proposed sequence is Asp1-Leu-Cys-Glu-Gln-Ser-Ala-Leu-Gln-Cys10-Asn-Glu-Gln-Gly-Cys-His -Asn-Phe-Cys- Ser20-Pro-Glu-Asp-Lys-Pro-Gly-Cys-Leu-Gly-Met30-Val-Trp-Asn- Pro-Glu-Leu-Cys- Pro38. The calculated molecular weight of 4191.7, which is in good agreement with the value of m/z 4190.7 obtained by fission fragment ionization mass spectrometry, suggests that the native structure is a dimer with three intrachain disulfide bonds in each subunit. The amino acid sequence is 43% identical with that of the E. raikovi mating pheromone Er-1, with the identities concentrated in the amino-terminal half. The half-cystine locations are conserved, but Er-10 is two residues shorter than Er-1. Prediction of the secondary structure suggests that Er-10 may also contain a helical structure at the a...Continue Reading

Citations

Jan 1, 1995·Developmental Genetics·A La TerzaP Luporini
Jun 1, 1992·Protein Science : a Publication of the Protein Society·A E StewartR A Bradshaw
Sep 1, 1994·Protein Science : a Publication of the Protein Society·S MrongaK Wüthrich
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S RaffioniR A Bradshaw
May 1, 1990·Trends in Genetics : TIG·G Beale
May 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J L RamJ D Hardege
Nov 5, 1990·Journal of Molecular Biology·D AndersonD Eisenberg

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