Purification, characterization and antiglycation activity of a novel polysaccharide from black currant

Food Chemistry
Yaqin XuJing Dai

Abstract

A novel polysaccharide fraction (BCP-1) was extracted from the black currant fruit by ultrasound-assisted compound enzyme and purified by chromatography on macroporous resin D4006, anion-exchange Q-Sepharose FF and Sephadex G-100 columns. BCP-1 consisted of galacturonic acid, xylose, mannose, glucose and galactose in a ratio of 1.00:3.14:1.83:17.90:1.98 and its molecular weight was 14,050Da. The preliminary structure features of BCP-1 were investigated by FT-IR and NMR. SEM and Congo red test showed that BCP-1 had honeycomb-like structure, but no triple helix structure. BCP-1 exhibited significant inhibitory abilities on protein glycation. Especially, BCP-1 showed obvious inhibitory effects on the formation of dicarbonyl compounds and AGEs (% inhibition of 66.95±0.33% and 67.15±0.40% respectively), but weaker inhibition Amadori compound production (max. 37.15±0.36% inhibition). This suggested that the inhibitory action of BCP-1 on protein glycation was more effective on the later phases of dicarbonyl compounds and AGEs formation.

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Citations

Mar 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Haifeng LiZebo Huang
Jul 30, 2019·Journal of Food Biochemistry·Shuhong LiYe Chen
Aug 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan RenAntonio Del Rio Flores
Jul 18, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Feng-Jie CuiDi Wu
Feb 8, 2020·International Journal of Biological Macromolecules·Xiaolong JiYanqi Liu
Jan 30, 2021·BioMed Research International·Imen TrabelsiRiadh Ben Salah
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yang TangYanhua Li
Mar 27, 2021·Environmental Technology·Fan YangYu Shen
Mar 22, 2021·International Journal of Biological Macromolecules·Khaled ArabKambiz Jahanbin
Oct 17, 2020·International Journal of Biological Macromolecules·Gülsen Tel-ÇayanMehmet Öztürk
Apr 20, 2021·International Journal of Biological Macromolecules·Honglin WangYuanqiang Guo
Jun 5, 2018·International Journal of Biological Macromolecules·Ines TriguiM A Ayadi
Aug 28, 2021·International Journal of Biological Macromolecules·Maosheng WeiQuanbin Zhang

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