Purification, characterization and antitumor activity of polysaccharides extracted from Phellinus igniarius mycelia

Carbohydrate Polymers
Shi-Chao LiDan-Zhao Guo

Abstract

Water-soluble intracellular polysaccharides (IPS) were extracted from cultured mycelia of Phellinus igniarius. The IPS were purified by ethanol fractional precipitation, ion-exchange and size exclusion chromatography in that order. Homogeneous polysaccharide IPSW-1, IPSW-2, IPSW-3, and IPSW-4 were obtained, which molecular characteristics were examined using multiangle laser-light scattering and refractive index detector system. The average molecular weights of them were 34.1, 17.7, 15.1, 21.7kDa, respectively. GC analysis indicated that IPSW-1, IPSW-2 and IPSW-3 all only contained glucose, while IPSW-4 was composed of rhamnose, xylose, mannose, glucose and galactose in a molar ratio of 1.29:1.21:1:43.86:1.86. UV and IR analysis suggested they belonged to α-type of the pyran group and didn't contain protein. These homogeneous polysaccharides could inhibit the growth of SW480 and HepG2 cells to a certain extent in a dose-dependent manner. So they could be beneficial for the further development of a natural carcinoma preventive agent and functional food.

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Citations

Aug 26, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Liangliang CaiLibiao Luan
Dec 29, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·An-Jun LiuHui-Ping Liu
Aug 14, 2020·Critical Reviews in Biotechnology·Luciana LuftMarcio A Mazutti
Jan 4, 2021·Bioprocess and Biosystems Engineering·Luciana LuftMarcio A Mazutti
Jan 20, 2021·International Journal of Biological Macromolecules·Prasenjit MaityGajendra Nath Maity
Jul 13, 2019·Biochemical and Biophysical Research Communications·Yanjie HouYurong Guo
Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Débora Cássia Vieira GomesAna Amélia de Carvalho Melo Cavalcante
Mar 20, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fei-Fei WangNing Li

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