Purification, characterization, and immunological cross-reactivity of alginates produced by mucoid Pseudomonas aeruginosa from patients with cystic fibrosis.

Journal of Clinical Microbiology
S S PedersenG H Shand

Abstract

Alginates from nine mucoid Pseudomonas aeruginosa isolates from patients with cystic fibrosis were purified by repeated ethanol precipitation, nuclease digestion, anion-exchange chromatography, dialysis, and lyophilization. Uronic acid constituted 72% of the dry weight when mannuronolactone was used as the internal standard in the carbazole-borate assay for uronic acids. The average degree of acetylation was 16%, and the ratio of mannuronic acid to gluluronic acid was 4.7. No homopolymeric blocks of guluronic acid were found when analyzed by nuclear magnetic resonance spectroscopy. Contaminating proteins were denatured by heating, and during purification the content of protein relative to alginate fell from 566 to 0.9%. The content of lipopolysaccharide was 0.012%. No immunological or biological activity was attributable to the protein or lipopolysaccharide content as estimated by immunoblotting, enzyme-linked immunosorbent assay (ELISA), and a neutrophil chemotaxis assay. Rabbits were hyperimmunized with P. aeruginosa alginates and alginate from the seaweed Laminaria hyperborea, and an ELISA that detected alginate-specific antibodies was developed. Antibodies to P. aeruginosa alginate were detected by ELISA in 1:4,000 dilution...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1986·Annual Review of Microbiology·N HøibyP O Schiøtz
Aug 1, 1987·Infection and Immunity·D B LearnS Seetharama
Oct 15, 1986·Carbohydrate Research·G Skjåk-BraekB Larsen
Feb 13, 1974·Journal of Chromatography·C Bucke
Sep 1, 1968·Analytical Biochemistry·C A Knutson, A Jeanes
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Dec 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Bussolati, P Gugliotta
Mar 1, 1983·The Journal of Infectious Diseases·G B PierD D Eardley
Sep 1, 1986·Applied and Environmental Microbiology·W F FettT S Siebles
Jun 1, 1971·Infection and Immunity·S Schwarzmann, J R Boring

❮ Previous
Next ❯

Citations

Feb 6, 1998·FEMS Microbiology Reviews·E S Stanislavsky, J S Lam
Mar 24, 2005·Infection and Immunity·Nadine HoffmannNiels Høiby
May 3, 2013·APMIS. Supplementum·Thomas Bjarnsholt
Nov 3, 2005·Journal of Biomedical Materials Research. Part a·Julie DusseaultJean-Pierre Hallé
Jun 22, 2007·Chemical Biology & Drug Design·Hsin H KuoCharles M Deber
Mar 10, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Wan C TsaiUmadevi Sajjan
Oct 17, 2012·Antimicrobial Agents and Chemotherapy·John W Lamppa, Karl E Griswold
Dec 1, 1992·The Journal of Applied Bacteriology·D G Allison, M J Matthews
May 7, 2009·Pediatric Pulmonology·Thomas BjarnsholtNiels Høiby
Mar 19, 2013·Applied Microbiology and Biotechnology·Hyeon Jin ChoiDong Suk Park
Dec 4, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ali FarjahHashem Khorsand Mohammadpour
Apr 12, 2016·Journal of Applied Microbiology·Leonardo A P GermoniIain L Lamont
Sep 4, 2020·Expert Opinion on Drug Discovery·Xiaolong ShaoXin Deng
May 30, 2008·Journal of Clinical Microbiology·Klaus Kirketerp-MøllerThomas Bjarnsholt
Jun 1, 1991·Applied and Environmental Microbiology·B J BrownL O Ingram

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.