Abstract
A polysaccharide CP1-1 was isolated and purified from Celosia cristata. Its average molecular weight was 2.3kDa and it was composed of glucose and galactose in a ratio of 1.00:2.03, and traces of mannose. Chemical characterization of CP1-1 was elucidated by methylation analysis. CP1-1 was a branched glucogalactan which was mainly composed of 1,6-linked Galp and 1,6-linked Glcp with a ratio of 5.6:3.8. The branches were at the O-3 of the main chain and might be composed of single terminal (1→)-linked glucopyranose and galactopyranose. CP1-1 also significantly promoted the proliferation and neutral red phagocytosis of RAW 264.7 macrophage cells in vitro. In addition, CP1-1 promoted cell proliferation by enhancing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and IL-1β. These results suggested that the polysaccharide from C. cristata could be used as a potential immunostimulator.
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Citations
Jul 16, 2016·Journal of the Science of Food and Agriculture·Daheng ZhengXiangyang Wu
Sep 1, 2016·Carbohydrate Polymers·Yuting TianBaodong Zheng
Feb 12, 2020·Toxicology·Mingjie ShiMing Shi
Feb 27, 2021·International Journal of Biological Macromolecules·Zheng ZhangYiqun Wan
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Jul 2, 2021·International Journal of Biological Macromolecules·Yan ZhouHongwei Li
Dec 12, 2021·The Journal of Pharmacy and Pharmacology·Yizhou LvYunpeng Fan