Purification, crystallization and preliminary X-ray analysis of the outer membrane complex HasA-HasR from Serratia marcescens

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Frédéric HuchéWolfram Welte

Abstract

Serratia marcescens is able to acquire iron using its haem-acquisition system (;has'), which contains an outer membrane receptor HasR and a soluble haemophore HasA. After secretion, HasA binds free haem in the extracellular medium or extracts it from haemoproteins and delivers it to the receptor. Here, the crystallization of a HasA-HasR complex is reported. HasA and HasR have been overexpressed in Escherichia coli and the complex formed and crystallized. Small platelets and bunches of needles of dimensions 0.01 x 0.1 x 1 mm were obtained. A native data set has been collected to 6.8 A.

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Citations

Jan 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Stefanie KriegWolfram Welte
Jan 25, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Erin Sarah Honsa, Anthony William Maresso
Oct 6, 2007·Biochimica Et Biophysica Acta·Karla D Krewulak, Hans J Vogel

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