Purification, crystallization and preliminary X-ray crystallographic analysis of rice lectin from Oryza sativa

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Yen-Chieh HuangChun-Jung Chen

Abstract

Lectins with sugar-binding specificity are widely distributed in higher plants and various other species. The expression of rice lectin from Oryza sativa is up-regulated in the growing coleoptile when anaerobic stress persists. A rice lectin of molecular weight 15.2 kDa has been crystallized using the hanging-drop vapour-diffusion method. From the diffraction of the lectin crystals at 1.93 A resolution, the unit cell belongs to space group P3(1), with unit-cell parameters a = 98.58, b = 98.58, c = 44.72 A. Preliminary analysis indicates that there are two lectin molecules in an asymmetric unit with a large solvent content, 70.1%.

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Citations

Jul 18, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hui-Fen LiaoYu-Jen Chen

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