Purification, crystallization and preliminary X-ray analysis of beta-glucosidase from Kluyveromyces marxianus NBRC1777.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Erina YoshidaHidehiko Kumagai

Abstract

The intracellular beta-glucosidase from Kluyveromyces marxianus NBRC1777 (KmBglI) belongs to glycoside hydrolase family 3 and has a unique domain architecture. Selenomethionine-labelled KmBglI was purified and crystallized by the hanging-drop vapour-diffusion method using the purified enzyme at 30 mg ml(-1), 0.04 M potassium dihydrogen phosphate pH 5.1, 16%(w/v) PEG 8000 and 20%(v/v) glycerol. The crystal belonged to space group C2, with unitcell parameters a = 245.8, b = 148.7, c = 119.9 angstrom, beta = 112.9 degrees. Multiple-wavelength anomalous dispersion data were collected at 2.4 and 2.5 angstrom resolution. A tetramer was assumed to be present in the asymmetric unit, which gave a Matthews coefficient of 2.6 angstrom(3) Da(-1).

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Nov 1, 1995·Protein Science : a Publication of the Protein Society·C N PaceT Gray
Jul 9, 2004·Trends in Biochemical Sciences·Daniel J RigdenMichael Y Galperin
Dec 31, 2005·Nucleic Acids Research·Robert D FinnAlex Bateman
Oct 13, 2006·Antonie van Leeuwenhoek·Antonius J A van MarisJack T Pronk
Apr 17, 2007·Journal of Biotechnology·Jiong HongHisanori Tamaki
Sep 5, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Pernilla TurnerDerek T Logan
Apr 23, 2008·Applied Microbiology and Biotechnology·Gustavo Graciano FonsecaAndreas K Gombert
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat

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Citations

Jan 7, 2014·Applied Biochemistry and Biotechnology·Mariana R LopesLuciano G Fietto
Apr 4, 2020·Current Pharmaceutical Design·Sarah Canal MaximinoWarley de Souza Borges
Jan 1, 2013·Biomolecules·Annette SørensenBirgitte K Ahring
Apr 20, 2021·Applied Biochemistry and Biotechnology·Ravish GodseRam Kulkarni

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