Purification, crystallization and preliminary X-ray diffraction analysis of a novel keto-deoxy-D-galactarate (KDG) dehydratase from Agrobacterium tumefaciens

Acta Crystallographica. Section F, Structural Biology Communications
Helena TabermanJuha Rouvinen

Abstract

D-galacturonic acid is the main component of pectin. It could be used to produce affordable renewable fuels, chemicals and materials through biotechnical conversion. Keto-deoxy-D-galactarate (KDG) dehydratase is an enzyme in the oxidative pathway of D-galacturonic acid in Agrobacterium tumefaciens (At). It converts 3-deoxy-2-keto-L-threo-hexarate to α-ketoglutaric semialdehyde. At KDG dehydratase was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 169.1, b = 117.8, c = 74.3 Å, β = 112.4° and an asymmetric unit of four monomers. X-ray diffraction data were collected to 1.9 Å resolution using synchrotron radiation. The three-dimensional structure of At KDG dehydratase will provide valuable information on the function of the enzyme and will allow it to be engineered for biorefinery-based applications.

References

Apr 1, 1970·Journal of Bacteriology·Y F Chang, D S Feingold
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
May 1, 1996·Analytical Biochemistry·J PeränenL Kääriäinen
Oct 1, 1958·Biochimica Et Biophysica Acta·A ASHWELLJ HICKMAN
May 16, 1959·Nature·W W KILGORE, M P STARR
Nov 1, 1959·Journal of Bacteriology·J E ZAJIC
Dec 8, 2007·The Biochemical Journal·Georgia KefalaRenwick C J Dobson
Mar 28, 2008·The Journal of Biological Chemistry·Asadollah AghaieAlain Perret
Jan 23, 2009·Applied Microbiology and Biotechnology·Peter Richard, Satu Hilditch
Nov 19, 2009·Applied Microbiology and Biotechnology·Harry BoerPeter Richard
Dec 2, 2009·The Journal of Biological Chemistry·Jarrod E VossMatthew A Perugini
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Alexei Vagin, Alexei Teplyakov
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Jun 17, 2011·The Journal of Biological Chemistry·Tarja ParkkinenJuha Rouvinen
Apr 20, 2012·Journal of Synchrotron Radiation·Daniele de SanctisChristoph Mueller-Dieckmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Ivana Leščić AšlerZoran Štefanić
Acta Crystallographica. Section D, Biological Crystallography
A DessenW Weissenhorn
Acta Crystallographica. Section D, Biological Crystallography
Yves PeeraerCamiel J De Ranter
Acta Crystallographica. Section D, Biological Crystallography
Balasundaram Padmanabhan, Masami Horikoshi
© 2022 Meta ULC. All rights reserved