Purification, crystallization and preliminary crystallographic analysis of the Hermes transposase

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Zhanita N PerezAlison Burgess Hickman

Abstract

DNA transposition is the movement of a defined segment of DNA from one location to another. Although the enzymes that catalyze transposition in bacterial systems have been well characterized, much less is known about the families of transposase enzymes that function in higher organisms. Active transposons have been identified in many insect species, providing tools for gene identification and offering the possibility of altering the genotypes of natural insect populations. One of these active transposons is Hermes, a 2749-base-pair element from Musca domestica that encodes its own transposase. An N-terminally deleted version of the Hermes transposase (residues 79-612) has been overexpressed and purified, and crystals that diffract to 2.1 A resolution have been obtained at 277 K by the hanging-drop method.

References

Sep 25, 2003·Insect Biochemistry and Molecular Biology·K MichelP W Atkinson
Apr 23, 2004·Current Opinion in Structural Biology·Mindy Steiniger-WhiteWilliam S Reznikoff
Apr 23, 2004·Acta Crystallographica. Section D, Biological Crystallography·Julia M RichardsonMalcolm D Walkinshaw
Jun 1, 2004·DNA and Cell Biology·Vladimir V Kapitonov, Jerzy Jurka
Aug 12, 2004·Nucleic Acids Research·Stephan WatkinsTitia K Sixma

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Citations

Mar 3, 2012·G3 : Genes - Genomes - Genetics·Lucie FouvryCatherine Montchamp-Moreau

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